通过负染色电子显微镜对大量病毒样本进行筛选的快速半定量方法。
Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy.
作者信息
Monroe J H, Brandt P M
出版信息
Appl Microbiol. 1970 Aug;20(2):259-62. doi: 10.1128/am.20.2.259-262.1970.
Abstract
A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.
摘要
本文介绍了一种通过负染色电子显微镜快速筛选大量病毒样本的半定量方法。将该方法获得的结果与通过假复制品法和定量滴度法获得的结果进行比较。给出了一个表示负染色法检测病毒的可检测极限的图表。
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本文引用的文献
1
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Virology. 1961 Dec;15:428-35. doi: 10.1016/0042-6822(61)90110-6.
2
Biological studies on a lymphoid-leukemia virus extracted from sarcoma 37. I. Origin and introductory investigations.从肉瘤37中提取的一种淋巴白血病病毒的生物学研究。I. 起源与初步研究。
J Natl Cancer Inst. 1960 Apr;24:933-51.
3
Electron densitometry of stained virus particles.染色病毒颗粒的电子密度测定法。
J Biophys Biochem Cytol. 1955 Jan;1(1):1-12. doi: 10.1083/jcb.1.1.1.
4
APPLICATION OF IMMUNOFLUORESCENCE TO THE STUDY OF HUMAN LEUKEMIA.免疫荧光在人类白血病研究中的应用
J Natl Cancer Inst. 1964 Sep;33:581-8.
5
CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA).伯基特氏瘤（非洲淋巴瘤）的细胞学
Lancet. 1964 Feb 1;1(7327):238-40. doi: 10.1016/s0140-6736(64)92345-1.
6
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Biochim Biophys Acta. 1962 Sep 17;61:321-31. doi: 10.1016/0926-6550(62)90132-9.
7
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