Bramucci M G, Keggins K M, Lovett P S
J Virol. 1977 Oct;24(1):194-200. doi: 10.1128/JVI.24.1.194-200.1977.
The pseudotemperate phage PMB12 was isolated from soil on the basis of its ability to enhance the rate of sporulation of Bacillus subtilis 168. PMB12 was subsequently shown to convert the sporulation defect in two genetically distinct classes of sporulation mutants. One class includes those rifampin-resistant mutants that are also spore-negative (mutated at the rif locus). The other class includes a strain carrying the sporulation mutation spoCM-1. The spoCM-1 mutation is linked to cysA15 by PBS1 transduction but is distinct from the rif locus. Several other sporulation mutants were not converted by PMB12. PMB12 is related to phage PBS1. However, PBS1 did not convert the above sporulation mutants. The replication of PBS2, a clear-plaquing derivative of PBS1, is rifampin insensitive, apparently due to a phage-induced rifampin-insensitive RNA polymerase. PMB12 replication is also rifampin insensitive.
假温和噬菌体PMB12是从土壤中分离出来的，其依据是它能够提高枯草芽孢杆菌168的芽孢形成率。随后发现PMB12能在两类遗传上不同的芽孢形成突变体中改变芽孢形成缺陷。一类包括那些对利福平耐药且也是孢子阴性的突变体（在利福平位点发生突变）。另一类包括携带芽孢形成突变spoCM-1的菌株。通过PBS1转导，spoCM-1突变与cysA15连锁，但与利福平位点不同。其他几个芽孢形成突变体未被PMB12转化。PMB12与噬菌体PBS1相关。然而，PBS1并未转化上述芽孢形成突变体。PBS1的一个清亮噬菌斑衍生物PBS2的复制对利福平不敏感，显然是由于一种噬菌体诱导的对利福平不敏感的RNA聚合酶。PMB12的复制对利福平也不敏感。
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