枯草芽孢杆菌核糖核酸聚合酶突变体的遗传分析。
Genetic analysis of ribonucleic acid polymerase mutants of Bacillus subtilis.
作者信息
Haworth S R, Brown L R
出版信息
J Bacteriol. 1973 Apr;114(1):103-13. doi: 10.1128/jb.114.1.103-113.1973.
Abstract
Deoxyribonucleic acid-dependent ribonucleic acid polymerase mutants of Bacillus subtilis strain Marburg were isolated after mutagenesis of spores with ethyl methane sulfonate. Genetic analysis by PBS1-mediated transduction and by transformation indicated that mutations responsible for all of the four phenotypic classes studied (rifampin resistance, streptovaricin resistance, streptolydigin resistance, and temperature sensitivity) were clustered close to the cysA14 locus. Three-factor transformation analysis has indicated the most probable marker order as follows: Rif(R)(Stv)(R)-Std(R)-Ts(418)-Ts(427). In addition, further characterization of the classical group I reference marker, cysA14, is reported.
摘要
用甲磺酸乙酯对枯草芽孢杆菌马尔堡菌株的孢子进行诱变后，分离得到了依赖脱氧核糖核酸的核糖核酸聚合酶突变体。通过PBS1介导的转导和转化进行的遗传分析表明，导致所研究的所有四种表型类别（利福平抗性、链霉杀阳菌素抗性、链霉溶菌素抗性和温度敏感性）的突变都聚集在cysA14位点附近。三因子转化分析表明最可能的标记顺序如下：Rif(R)(Stv)(R)-Std(R)-Ts(418)-Ts(427)。此外，还报道了经典的I类参考标记cysA14的进一步特征。
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