Lovett P S, Young F E
J Bacteriol. 1970 Feb;101(2):603-8. doi: 10.1128/jb.101.2.603-608.1970.
Bacteriophage PBS1 mediates generalized transduction in Bacillus pumilus NRRL B-3275 (BpB1). Transduction frequencies for single auxotrophic markers are of the order of 10(-4) transductants per plaque-forming unit in crude phage lysates. The characteristics of PBS1 propagated on BpB1 and the properties of the system of transduction are similar to those reported for PBS1 propagated on Bacillus subtilis. By transduction, eight amino acid auxotrophic markers in BpB1 have been oriented into two linkage groups. One group contains the auxotrophic markers arginine A, leucine, and phenylalanine, and the other group contains the markers lysine, serine, tryptophan, isoleucine-valine, and isoleucine. The nature and relative order of the markers within each linkage group suggest that the arrangement of genes in these areas of the chromosome of BpB1 is similar to the arrangement of phenotypically comparable genes in two linkage groups (defined by PBS1 transduction) in B. subtilis. However, transduction of any of the above cited markers in BpB1 to prototrophy with PBS1 propagated on B. subtilis 168 could not be demonstrated. In addition to BpB1, seven other strains of B. pumilus can be infected with PBS1. Transduction has been demonstrated in three of these strains.
噬菌体PBS1介导短小芽孢杆菌NRRL B - 3275（BpB1）中的普遍性转导。在粗噬菌体裂解物中，单个营养缺陷型标记的转导频率约为每噬菌斑形成单位10^(-4)个转导子。在BpB1上繁殖的PBS1的特性以及转导系统的性质与在枯草芽孢杆菌上繁殖的PBS1所报道的相似。通过转导，已将BpB1中的八个氨基酸营养缺陷型标记定位到两个连锁群中。一个连锁群包含精氨酸A、亮氨酸和苯丙氨酸营养缺陷型标记，另一个连锁群包含赖氨酸、丝氨酸、色氨酸、异亮氨酸 - 缬氨酸和异亮氨酸标记。每个连锁群内标记的性质和相对顺序表明，BpB1染色体这些区域中的基因排列与枯草芽孢杆菌中两个连锁群（由PBS1转导定义）中表型可比基因的排列相似。然而，未证明用在枯草芽孢杆菌168上繁殖的PBS1将BpB1中的上述任何标记转导为原养型。除了BpB1之外，其他七种短小芽孢杆菌菌株也可被PBS1感染。已在其中三种菌株中证明了转导。
