Karavodin L M, Ash L R
Clin Exp Immunol. 1980 May;40(2):312-7.
Circulating immune complexes have been investigated in jirds (Meriones unguiculatus) infected with the filarial nematode Brugia pahangi. Two-month-old male jirds were inoculated with seventy-five B. pahangi infective larvae into the left groin. At 8 months post-infection, sera of individual animals from a group of seventeen infecteds and seventeen age-matched controls were analysed for immune complexes by (1) a solid-phase C1q binding assay (Clq-SP) and (2) precipitation with 3.5% polyethylene glycol followed by binding of 125I-labelled rabbit anti-jird Ig antiserum (PEG). A significant increase in the level of circulating immune complexes was shown in the infected group as compared with the controls for both assays, with a P value = 0.005 for PEG and P = 0.001 for Clq-SP. Using the mean of the control group +/- 2 s.d. as the upper limit of the normal range, 24% of the infected group had elevated immune complex levels by the PEG assay, and 41% were elevated in the C1q-SP assay. A high degree of variability was noted in the levels of immune complexes among individual animals in the infected group by each test. No correlation between immune complex levels and numbers of circulating microfilariae was found in either assay.
在感染了丝状线虫彭亨布鲁线虫（Brugia pahangi）的沙鼠（Meriones unguiculatus）中对循环免疫复合物进行了研究。将75条彭亨布鲁线虫感染性幼虫接种到2月龄雄性沙鼠的左腹股沟。在感染后8个月，通过（1）固相C1q结合试验（Clq-SP）和（2）用3.5%聚乙二醇沉淀，随后结合125I标记的兔抗沙鼠Ig抗血清（PEG），对一组17只感染动物和17只年龄匹配的对照动物的个体血清进行免疫复合物分析。两种试验均显示，与对照组相比，感染组的循环免疫复合物水平显著升高，PEG试验的P值 = 0.005，Clq-SP试验的P = 0.001。以对照组平均值±2个标准差作为正常范围的上限，PEG试验显示24%的感染组免疫复合物水平升高，C1q-SP试验中41%升高。通过每项试验发现，感染组个体动物的免疫复合物水平存在高度变异性。两种试验均未发现免疫复合物水平与循环微丝蚴数量之间存在相关性。
