利用拉吉细胞通过酶联免疫吸附测定法对血清中循环免疫复合物进行定量分析。
Quantitation of circulating immune complexes in serum by Raji cells using an enzyme-linked immunosorbent assay.
作者信息
Cunningham-Rundles C, Brandeis W E, Zacharczuk T, Good R A, Day N K
出版信息
Clin Exp Immunol. 1980 May;40(2):411-5.
An enzyme-linked immunosorbent assay (ELISA) for the quantitation of immune complexes in sera has been developed using Raji cells. In this assay the Raji cell-adherent complexes are detected by an alkaline phosphatase-conjugated rabbit anti-human IgG and the results are obtained by spectrophotometric measurements of the concentration of yellow colour produced after the enzyme's substrate, p-nitrophenyl phosphate, is added. This assay is as sensitive and reproducible as the Raji cell radioimmune assay using 125I, as it can detect as little as 16 micrograms of immune complex equivalent to heat-aggregated IgG/ml in serum samples. This method is inexpensive, convenient and most importantly, avoids the biohazards of using an isotope.
已开发出一种使用拉吉细胞定量血清中免疫复合物的酶联免疫吸附测定（ELISA）法。在此测定中，通过碱性磷酸酶偶联的兔抗人IgG检测拉吉细胞黏附的复合物，并在加入酶的底物对硝基苯磷酸后，通过分光光度法测量产生的黄色浓度来获得结果。该测定与使用125I的拉吉细胞放射免疫测定一样灵敏且可重复，因为它能检测出血清样品中低至16微克相当于热聚集IgG/ml的免疫复合物。该方法成本低廉、操作简便，最重要的是，避免了使用同位素带来的生物危害。
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