Li Meiqi, Li Jingling, An Zehao, Wang Shasha, Lai Youpeng
Qinghai Provincial Key Laboratory of Comprehensive Management of Agricultural Pests, Qinghai University, Xining 810016, China.
Plant Protection Institute, Qinghai Academy of Agricultural and Forestry Sciences, Xining 810016, China.
Biology (Basel). 2025 Sep 10;14(9):1240. doi: 10.3390/biology14091240.
, a significant pest of highland spring wheat, damages crops through larval boring and feeding within wheat stalks. This activity disrupts nutrient and water transport, causing severe yield reductions. To find microbial biocontrol agents targeting this pest, primary entomopathogenic microorganisms were isolated and identified from naturally infected, deceased larvae. Morphological examination and ITS-based phylogenetic analysis tentatively identified the isolate as the entomopathogenic fungus sp. (strain CF01). Third-instar larvae of were inoculated with conidial suspensions of the CF01 strain at concentrations of 1 × 10, 1 × 10, 1 × 10, and 1 × 10 spores/mL. Spore suspensions of different concentrations demonstrated pathogenicity against third-instar larvae of The optimal growth conditions for strain CF01 were identified as follows: PPDA medium, 25 °C, fructose as the carbon source, and yeast extract as the nitrogen source. Photoperiod exhibited no significant effect on either mycelial growth or sporulation. These findings indicate that the CF01 strain possesses considerable potential for the biocontrol of .
作为高原春小麦的一种重要害虫，通过幼虫在小麦茎秆内钻孔和取食来损害作物。这种活动会扰乱养分和水分运输，导致产量大幅下降。为了找到针对这种害虫的微生物生物防治剂，从自然感染死亡的幼虫中分离并鉴定了主要的昆虫病原微生物。形态学检查和基于ITS的系统发育分析初步将该分离物鉴定为昆虫病原真菌 种（菌株CF01）。用浓度为1×10、1×10、1×10和1×10孢子/毫升的CF01菌株分生孢子悬浮液接种 的三龄幼虫。不同浓度的孢子悬浮液对 的三龄幼虫均表现出致病性。CF01菌株的最佳生长条件确定如下：PPDA培养基、25℃、果糖作为碳源、酵母提取物作为氮源。光周期对菌丝生长和孢子形成均无显著影响。这些发现表明CF01菌株在生物防治 方面具有相当大的潜力。
