Dornbusch K
Scand J Infect Dis. 1977;9(3):227-31. doi: 10.3109/inf.1977.9.issue-3.14.
Assay of gentamicin concentrations were performed on 167 patient sera by two methods in the routine work. A radioenzymatic method that measures the acetylation of getamicin by a bacterial enzyme extract and the overnight agar diffusion method, using cups as diffusion centres, were used. The methods correlated well (r = 0.87), although the results of the microbiological assay in some cases were higher than the results of the enzymatic assay. When artificial samples were tested the microbiological methods gave variable results. Therefore careful standardization is necessary for accuracy and reproducibility. The enzymatic assay, however, offered advantages of specificity, reproducibility and rapidity.
在日常工作中，采用两种方法对167份患者血清进行庆大霉素浓度检测。一种是放射酶法，通过细菌酶提取物来测定庆大霉素的乙酰化程度；另一种是过夜琼脂扩散法，以杯状物作为扩散中心。尽管在某些情况下微生物检测法的结果高于酶法检测结果，但两种方法的相关性良好（r = 0.87）。对人工样本进行检测时，微生物检测法的结果不稳定。因此，为确保准确性和可重复性，需要仔细进行标准化操作。然而，酶法检测具有特异性、可重复性和快速性等优点。
