Płachno Bartosz J, Kapusta Małgorzata, Feldo Marcin, Stolarczyk Piotr, Świątek Piotr
Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University, 9 Gronostajowa St., 30-387 Kraków, Poland.
Bioimaging Laboratory, Faculty of Biology, University of Gdańsk, 59 Wita Stwosza St., 80-308 Gdańsk, Poland.
Int J Mol Sci. 2025 Sep 19;26(18):9174. doi: 10.3390/ijms26189174.
Glands from pitcher secrete various substances, including digestive enzymes, and absorb nutrients from digested prey. Due to the extreme specialization of these glands, they are an interesting model for studying secretory cells' structure and activity. This study aimed to fill the gap in the literature concerning the immunocytochemistry of digestive glands in the major cell wall polysaccharides and glycoproteins. To do this, the localization of the cell wall components in the cell walls of glandular cells was performed using whole-mount immunolabeled glands of . Also, we wanted to check to what extent the cuticles of glandular cells with discontinuities would be a barrier to the antibodies. The technique used allowed for the localization of de-esterified pectic homogalacturonans in the outer walls of gland cells. The remaining antibodies (which detect esterified pectins, hemicelluloses, and arabinogalactan protein) marked only debris or secretion residues on the gland or epidermal surfaces. Positive labeling with LM19 and CCRC-M38 antibodies suggests the presence of pectic homogalacturonan in the very superficial part of the glands' cell walls, so they were easily accessible to antibodies.
猪笼草的腺体分泌多种物质，包括消化酶，并从被消化的猎物中吸收营养。由于这些腺体的极端特化，它们是研究分泌细胞结构和活性的一个有趣模型。本研究旨在填补有关主要细胞壁多糖和糖蛋白中消化腺免疫细胞化学的文献空白。为此，使用猪笼草腺体的整装免疫标记标本对腺细胞细胞壁中的细胞壁成分进行定位。此外，我们想检查具有间断性的腺细胞角质层在多大程度上会成为抗体的屏障。所采用的技术能够定位腺细胞外壁中去酯化的果胶同型半乳糖醛酸聚糖。其余抗体（检测酯化果胶、半纤维素和阿拉伯半乳聚糖蛋白）仅标记了腺体或表皮表面的碎片或分泌残留物。用LM19和CCRC - M38抗体进行的阳性标记表明在腺体细胞壁的非常浅表部分存在果胶同型半乳糖醛酸聚糖，因此它们很容易被抗体识别。
