Solmaz Avcikurt Ayla, Adilay Huseyin Utku, Gunaldi Omur, Gultekin Tosun Sinem, Katar Salim
Department of Medical Biology, Faculty of Medicine, Balikesir University, Balikesir 10145, Turkey.
Department of Neurosurgery, Faculty of Medicine, Balikesir University, Balikesir 10145, Turkey.
Int J Mol Sci. 2025 Sep 20;26(18):9194. doi: 10.3390/ijms26189194.
In light of the growing significance of molecular biomarkers in central nervous system tumours, in this study, we aimed to comprehensively and quantitatively analyze the mRNA expression levels of (Parkinsonism-associated deglycase 7, ), (Growth Differentiation Factor 15), and (Milk Fat Globule-EGF Factor 8 Protein) in glioma and meningioma tissues and to thoroughly evaluate the associations between these gene expression profiles and clinicopathological parameters. Real-time PCR (qRT-PCR) analyses performed on tumour tissues obtained from a total of 27 glioma and 18 meningioma patients revealed that these three genes exhibited significantly elevated expression compared to control samples. Despite their different cellular origins, statistically significant positive correlations were observed between the expression levels of , , and and both tumour grade and the Ki-67 proliferation index (Ki-67 Pi) in both glioma and meningioma cases, indicating that higher gene expression is associated with increased tumour aggressiveness in both tumour types. Receiver operating characteristic (ROC) curve analyses further confirmed the diagnostic and prognostic potential of these genes. Additionally, protein-protein interaction networks involving the target genes were characterised, providing valuable insights into their molecular mechanisms. These findings suggest that , , and may play a role in the aggressiveness, invasion, and proliferation of gliomas and meningiomas. Moreover, integrating these genes as molecular biomarkers into tumour classification systems may provide a foundation for the development of personalised and targeted therapeutic strategies, although further studies are needed to support this.
鉴于分子生物标志物在中枢神经系统肿瘤中的重要性日益增加，在本研究中，我们旨在全面、定量地分析帕金森病相关去糖基化酶7（Parkinsonism-associated deglycase 7，DJ-1）、生长分化因子15（Growth Differentiation Factor 15，GDF15）和乳脂肪球表皮生长因子8蛋白（Milk Fat Globule-EGF Factor 8 Protein，MFGE8）在胶质瘤和脑膜瘤组织中的mRNA表达水平，并深入评估这些基因表达谱与临床病理参数之间的关联。对总共27例胶质瘤患者和18例脑膜瘤患者的肿瘤组织进行的实时PCR（qRT-PCR）分析显示，与对照样本相比，这三个基因的表达显著升高。尽管它们的细胞起源不同，但在胶质瘤和脑膜瘤病例中，DJ-1、GDF15和MFGE8的表达水平与肿瘤分级和Ki-67增殖指数（Ki-67 Pi）之间均观察到具有统计学意义的正相关，表明较高的基因表达与这两种肿瘤类型的侵袭性增加有关。受试者工作特征（ROC）曲线分析进一步证实了这些基因的诊断和预后潜力。此外，还对涉及靶基因的蛋白质-蛋白质相互作用网络进行了表征，为其分子机制提供了有价值的见解。这些发现表明，DJ-1、GDF15和MFGE8可能在胶质瘤和脑膜瘤的侵袭性、侵袭和增殖中发挥作用。此外，将这些基因作为分子生物标志物纳入肿瘤分类系统可能为个性化和靶向治疗策略的开发提供基础，尽管还需要进一步的研究来支持这一点。
