Sweney L R, Shapiro B L
Stain Technol. 1977 Jul;52(4):221-7. doi: 10.3109/10520297709116779.
We have developed a relatively rapid glutaraldehyde-tannic acid (GTA) and osmium tetroxide (OsO4) fixation procedure which permits many types of uncoated biological specimens to be examined in the scanning electron microscope (SEM) at 20 kV without the occurrence of charging. Most specimens taken one day can be examined in the SEM the following afternoon. Types of specimens successfully treated were perfused adult and embryonic rat tissues, confluent human skin fibroblast tissue cultures, plant roots, flowers, seeds, some garden insects, and microcolonies of salivary streptococci. Cells in suspension and extracted human teeth did become electron conductive when treated with the GTA procedure. Most suspended cells must be centrifuged between each solution and the GTA procedure increases the preparation time for these cells. Extracted teeth are usually simply dried and coated. Therefore, the usual SEM preparation techniques are shorter and perhaps more useful for these types of specimens.
我们开发了一种相对快速的戊二醛 - 单宁酸（GTA）和四氧化锇（OsO4）固定程序，该程序允许在扫描电子显微镜（SEM）中于20 kV下检查多种类型的未镀膜生物标本，而不会产生电荷。大多数在一天采集的标本可在次日下午用扫描电子显微镜检查。成功处理的标本类型包括灌注的成年和胚胎大鼠组织、汇合的人皮肤成纤维细胞组织培养物、植物根、花、种子、一些园林昆虫以及唾液链球菌的微菌落。当用GTA程序处理时，悬浮细胞和提取的人牙齿确实变得具有电子导电性。大多数悬浮细胞在每种溶液处理之间必须进行离心，并且GTA程序增加了这些细胞的制备时间。提取的牙齿通常只需干燥并镀膜。因此，常规的扫描电子显微镜制备技术更短，可能对这些类型的标本更有用。
