Suh Jeongmin, Ham Sehyeong, Na Hyejin, You Youngkook, Park Bumsoo, Chae Chanhee
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 08826, Republic of Korea.
CARESIDE Co., Ltd., Seongnam-si 13209, Republic of Korea.
Vaccines (Basel). 2025 Sep 5;13(9):951. doi: 10.3390/vaccines13090951.
The efficacy of a novel combined vaccine targeting porcine circovirus types 2a/d (PCV2a/d), , and was evaluated in a controlled challenge study.
A total of 45 pigs were randomly allocated into nine groups (five pigs per group). Vaccinated groups received a single 2 mL intramuscular dose of the combined vaccine and were subsequently challenged with PCV2a, PCV2d, , and . Unvaccinated groups received a single 2 mL intramuscular dose of phosphate-buffered saline (0.01 M, pH 7.4). Growth performance, systemic adaptive immune (humoral and cellular) responses, viremia, laryngeal and nasal mycoplasma loads, and histopathological lesions were assessed.
Vaccinated pigs exhibited enhanced growth performance and elicited systemic immune responses, including both humoral and cellular immunity, against all four pathogens. Vaccination also significantly reduced viremia, mycoplasmal loads in laryngeal and nasal swabs, and the severity of associated lesions compared with unvaccinated controls.
These results indicated that the combined vaccine was efficacious and conferred protection against PCV2a, PCV2d, , and challenge under experimental conditions. This combined vaccine represented an effective strategy to enhance growth performance and control complex co-infection in swine populations.
在一项对照攻毒研究中评估了一种针对2a/d型猪圆环病毒（PCV2a/d）等的新型联合疫苗的效力。
总共45头猪被随机分为9组（每组5头猪）。接种疫苗组肌肉注射单剂量2 mL联合疫苗，随后用PCV2a、PCV2d等进行攻毒。未接种疫苗组肌肉注射单剂量2 mL磷酸盐缓冲盐水（0.01 M，pH 7.4）。评估生长性能、全身适应性免疫（体液和细胞）反应、病毒血症、喉和鼻支原体载量以及组织病理学损伤。
接种疫苗的猪生长性能增强，并引发针对所有四种病原体的全身免疫反应，包括体液免疫和细胞免疫。与未接种疫苗的对照组相比，接种疫苗还显著降低了病毒血症、喉和鼻拭子中的支原体载量以及相关病变的严重程度。
这些结果表明，在实验条件下，联合疫苗有效并能提供针对PCV2a、PCV2d等攻毒的保护。这种联合疫苗是提高猪群生长性能和控制复杂混合感染的有效策略。
