Meers Paul
School of Environmental and Biological Sciences, 272 Foran Hall, Rutgers, The State University of New Jersey, 59 Dudley Rd., New Brunswick, NJ 08901-8250, USA.
Pharmaceutics. 2025 Sep 22;17(9):1231. doi: 10.3390/pharmaceutics17091231.
This brief review is a non-comprehensive look at some of the important aspects of lipidic nucleic acid delivery systems with a focus on RNA. In the context of this review on lipid-based formulation, nucleic acids are one of the key cargoes. Here, a brief historical background is given, highlighting a few of the key newly developing approaches to aid formulation design. These new techniques are discussed within a framework of "bottom-up" (rational) versus "top-down" (combinatorial) design. Evolving areas of interest that are discussed include multiplexed formulation and efficacy testing, new principles established in the role of the protein corona, details of the biophysical mechanism of delivery and machine learning approaches to design.
本简要综述并非对脂质核酸递送系统的所有重要方面进行全面审视，而是聚焦于RNA。在本次关于基于脂质的制剂的综述背景下，核酸是关键的运载物之一。在此，给出简要的历史背景，重点介绍一些有助于制剂设计的关键新发展方法。这些新技术在“自下而上”（理性）与“自上而下”（组合）设计的框架内进行讨论。所讨论的不断发展的感兴趣领域包括多重制剂和功效测试、蛋白质冠层作用中确立的新原理、递送生物物理机制的细节以及用于设计的机器学习方法。
