Chen Yunyi, Luo Shimei, Lei Nijing, Ye Zhenghao, Ma Xianping, Yang Shaoyu, Jia Huaijie, Qi Guangyu, Wang Guanghua, Yi Huashan
College of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China.
Guangxi Key Laboratory of Marine Environmental Disaster Processes and Ecological Protection Technology, Beibu Gulf University, Qinzhou 535011, China.
Vet Sci. 2025 Sep 13;12(9):886. doi: 10.3390/vetsci12090886.
Bluetongue virus belongs to the Reoviridae family, which causes morbidity in ruminants. Herein, we report the full-length genome sequence of a recombinant BTV strain of serotype 1 (YNDH/103/2013) isolated from sentinel cattle in Yunnan Province, China, in 2013. To identify and understand the characteristics of YNDH/103/2013, we screened closely related viruses using Simplot 3.5.1 software and conducted verification via phylogenetic analysis using MEGA 11.0.13 software. Phylogenetic analysis and segment examination showed that the YNDH/103/2013 strain likely originated from multiple recombination events involving prevalent strains such as Y863, NRT37/ABT/HSR, and YTS-4 in China and India. The genome of BTV-1/YNDH/103/2013 derives from at least three reassortments with these strains, showing recombination breakpoints mainly in segments 4, 6, 8, and 9. This study improves our understanding of the origin and spread of BTV-1 in China.
蓝舌病病毒属于呼肠孤病毒科，可引起反刍动物发病。在此，我们报告了2013年从中国云南省哨兵牛中分离出的1型重组蓝舌病病毒株（YNDH/103/2013）的全长基因组序列。为了鉴定和了解YNDH/103/2013的特征，我们使用Simplot 3.5.1软件筛选了密切相关的病毒，并使用MEGA 11.0.13软件通过系统发育分析进行了验证。系统发育分析和片段检查表明，YNDH/103/2013毒株可能起源于涉及中国和印度的Y863、NRT37/ABT/HSR和YTS-4等流行毒株的多次重组事件。BTV-1/YNDH/103/2013的基因组源自与这些毒株的至少三次重配，显示重组断点主要在第4、6、8和9片段。本研究提高了我们对BTV-1在中国的起源和传播的理解。
