Yousten A A, Hanson R S
J Bacteriol. 1972 Feb;109(2):886-94. doi: 10.1128/jb.109.2.886-894.1972.
A mutant of Bacillus subtilis 168 lacking aconitase (EC 4.2.1.3) was found to be blocked at stage 0 or I of sporulation. Although adenosine triphosphate levels, which normally decrease in tricarboxylic acid cycle mutants at the completion of exponential growth, could be maintained at higher levels by feeding metabolizable carbon sources, this did not permit the cells to progress further into the sporulation sequence. When post-exponential-phase cells of mutants blocked in the first half of the tricarboxylic acid cycle were resuspended with an energy source in culture fluid from post-exponential-phase wild-type B. subtilis or Escherichia coli, good sporulation occurred. The spores produced retained the mutant genotype and were heat stable but lost refractility and heat stability several hours after their production.
发现枯草芽孢杆菌168的一个缺乏乌头酸酶（EC 4.2.1.3）的突变体在芽孢形成的0期或I期受阻。尽管在指数生长完成时，三羧酸循环突变体中通常会降低的三磷酸腺苷水平可以通过供给可代谢碳源维持在较高水平，但这并不能使细胞在芽孢形成序列中进一步发展。当处于三羧酸循环前半段受阻的突变体的指数生长后期细胞与能量源一起重悬于来自指数生长后期野生型枯草芽孢杆菌或大肠杆菌的培养液中时，会发生良好的芽孢形成。产生的芽孢保留了突变体基因型，并且热稳定，但在产生后数小时失去折光性和热稳定性。
