聚-L-赖氨酸与染色质的相互作用。对大肠杆菌RNA聚合酶介导的原位RNA合成的抑制作用。
Interaction of poly-L-lysine with chromatin. Inhibition of in situ RNA synthesis mediated by Escherichia coli RNA polymerase.
作者信息
Umiel N, Plaut W
出版信息
J Cell Biol. 1973 Jan;56(1):139-44. doi: 10.1083/jcb.56.1.139.
RNA polymerase from Escherichia coli was used in conjunction with labeled nucleosides as an autoradiographic reagent to study the availability of template in the chromatin of fixed nuclei and chromosomes Sequential treatments of the tissues with acid and poly-L-lysine were used to compare the effect of these treatments on the availability of template with the previously reported effects on the in situ priming for Escherichia coli DNA polymerase Acid treatment was found to increase the in situ activity of both enzymes, while poly-L-lysine strongly inhibited the in situ reactions mediated by RNA and DNA polymerases. When the DNA polymerase reaction was previously carried out on alcohol-fixed chicken blood smears, leukocyte nuclei primed extensively for DNA synthesis. In contrast, we did not detect incorporation into intact nuclei of any cell type in alcohol-fixed blood smears that were treated with RNA polymerase.
来自大肠杆菌的RNA聚合酶与标记的核苷一起用作放射自显影试剂，以研究固定细胞核和染色体染色质中模板的可用性。用酸和聚-L-赖氨酸对组织进行顺序处理，以比较这些处理对模板可用性的影响与先前报道的对大肠杆菌DNA聚合酶原位引发的影响。发现酸处理可增加两种酶的原位活性，而聚-L-赖氨酸强烈抑制RNA和DNA聚合酶介导的原位反应。当DNA聚合酶反应先前在酒精固定的鸡血涂片上进行时，白细胞核广泛地引发DNA合成。相比之下，在用RNA聚合酶处理的酒精固定血涂片中，我们未检测到任何细胞类型的完整细胞核中有掺入现象。
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