染色质的结构。
The structure of chromatin.
作者信息
Mirsky A E
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2945-8. doi: 10.1073/pnas.68.12.2945.
Abstract
The DNA in chromatin of isolated thymus nuclei, and in two different preparations of deoxyribonucleoprotein extracted from chromatin, has been digested with DNase. By variation of the time of reaction and the concentration of enzyme, and by comparison of the action of the enzyme on (protein-free) DNA with its action on DNA complexed with protein in chromatin and deoxyribonucleoprotein, it was found that most, if not all, of the DNA is linked to protein. The linkage is loose, as shown by the fact that all the DNA is accessible to the enzyme; however, the wide variations in accessibility in a given complex show that the looseness of the DNA-protein link varies considerably. Accessibility of DNA in one type of deoxyribonucleoprotein preparation is greater than it is in chromatin, while in the other type it is less than in chromatin.
摘要
从胸腺中分离出的细胞核染色质中的DNA，以及从染色质中提取的两种不同的脱氧核糖核蛋白制剂中的DNA，均已用脱氧核糖核酸酶进行了消化。通过改变反应时间和酶浓度，并比较该酶对（无蛋白质的）DNA的作用与其对染色质和脱氧核糖核蛋白中与蛋白质复合的DNA的作用，发现大部分（如果不是全部的话）DNA与蛋白质相连。这种连接是松散的，因为所有的DNA都能被酶接触到；然而，在给定的复合物中可及性的广泛变化表明，DNA-蛋白质连接的松散程度差异很大。在一种类型的脱氧核糖核蛋白制剂中，DNA的可及性大于染色质中的，而在另一种类型中则小于染色质中的。
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本文引用的文献
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Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the reaction.结晶脱氧核糖核酸酶；胸腺核酸的消化；反应动力学
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A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
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