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不同布鲁氏菌属物种与小肠结肠炎耶尔森菌之间的血清学交叉反应。免疫扩散和免疫电泳。

Serological cross-reactions between different Brucella species and Yersinia enterocolitica. Immunodiffusion and immunoelectrophoresis.

作者信息

Hurvell B

出版信息

Acta Vet Scand. 1972;13(4):472-83. doi: 10.1186/BF03547153.

Abstract

By immunodiffusion and immunoelectrophoresis tests in agarose serological cross-reactions were demonstrated between Yersinia enterocolitica type IX and Brucella strains from four species (Brucella abortus, Brucella melitensis, Brucella suis and Brucella neotomae). No qualitative differences between these strains in their tendencies to cross-react with Yersinia enterocolitica type IX were observed. Brucella canis and Brucella ovis, which have nonsmooth colonial morphology, gave no demonstrable cross-reaction with Yersinia enterocolitica type IX. The results of absorption tests and qualitative staining reaction of the obtained precipitation lines suggest that the antigenic determinants common to Brucella and Yersinia enterocolitica type IX seemed to be associated with the outer layer and in the lipopolysaccharide complex of the respective bacteria. By immunodiffusion and immunoelectrophoresis it was possible to identify in hyperimmune sera those antibodies that derive from Brucella and Yersinia enterocolitica type IX.

摘要

通过琼脂糖中的免疫扩散和免疫电泳试验,证明了IX型小肠结肠炎耶尔森菌与来自四个种(流产布鲁菌、羊布鲁菌、猪布鲁菌和新墨西哥布鲁菌)的布鲁菌菌株之间存在血清学交叉反应。未观察到这些菌株在与IX型小肠结肠炎耶尔森菌交叉反应倾向方面的定性差异。具有非光滑菌落形态的犬布鲁菌和绵羊布鲁菌与IX型小肠结肠炎耶尔森菌未表现出可证实的交叉反应。吸收试验结果和所获沉淀线的定性染色反应表明,布鲁菌和IX型小肠结肠炎耶尔森菌共有的抗原决定簇似乎与各自细菌的外层和脂多糖复合物有关。通过免疫扩散和免疫电泳,可以在超免疫血清中鉴定出来自布鲁菌和IX型小肠结肠炎耶尔森菌的抗体。

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