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用于分离抗甘露聚糖特异性抗体的免疫吸附剂：酵母细胞壁中抗原的定位

Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.

作者信息

Gerber H, Horisberger M, Bauer H

出版信息

Infect Immun. 1973 Mar;7(3):487-92. doi: 10.1128/iai.7.3.487-492.1973.

DOI:10.1128/iai.7.3.487-492.1973
PMID:4123394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC422705/
Abstract

A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.

摘要

介绍了一种通过包埋在聚丙烯酰胺凝胶中来使酵母甘露聚糖不溶解的方法。通过免疫吸附从兔抗全福尔马林灭活产朊假丝酵母细胞血清的所有其他抗体中分离出特异性抗甘露聚糖抗体。用抗甘露聚糖和抗非甘露聚糖抗体组分包被的胶体金用于细胞壁抗原的超微结构可视化。使用正常兔血清进行对照。用甘露寡糖可抑制抗甘露聚糖抗体，且没有一个抗体组分显示出对酿酒酵母细胞壁的标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ea/422705/36f717960efd/iai00255-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ea/422705/3e619b98e4ec/iai00255-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ea/422705/386127f52037/iai00255-0175-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ea/422705/36f717960efd/iai00255-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ea/422705/3e619b98e4ec/iai00255-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ea/422705/386127f52037/iai00255-0175-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ea/422705/36f717960efd/iai00255-0176-a.jpg

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本文引用的文献

1
ANTIGENS AND ENZYMES MADE INSOLUBLE BY ENTRAPPING THEM INTO LATTICES OF SYNTHETIC POLYMERS.通过将抗原和酶包埋于合成聚合物晶格中使其不溶。
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Comparison of the effects of various detergents on antigen-antibody interaction.各种去污剂对抗抗原-抗体相互作用的影响比较。
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Mercury-labelled concanavalin A as a marker in electron microscopy-localisation of mannan in yeast cell walls.汞标记的伴刀豆球蛋白A作为电子显微镜下酵母细胞壁中甘露聚糖定位的标记物。
Experientia. 1975 Oct 15;31(10):1147-9. doi: 10.1007/BF02326761.
4
Localization of alpha-Galactomannan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy.通过扫描电子显微镜对α-半乳甘露聚糖在粟酒裂殖酵母细胞表面的定位
Arch Microbiol. 1977 Mar 1;112(2):123-6. doi: 10.1007/BF00429323.
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Localization of mannan at the surface of yeast protoplasts by scanning electron microscopy.通过扫描电子显微镜对酵母原生质体表面甘露聚糖进行定位
Arch Microbiol. 1976 Aug;109(1-2):9-14. doi: 10.1007/BF00425106.
6
Location of mannan and chitin on thin sections of budding yeasts with gold markers.用金标记物在出芽酵母薄片上定位甘露聚糖和几丁质。
Arch Microbiol. 1977 Oct 24;115(1):1-7. doi: 10.1007/BF00427837.
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Phosphomannans and other components of flocculent and non-flocculent walls of Saccharomyces cerevisiae.酿酒酵母絮凝型和非絮凝型细胞壁中的磷酸甘露聚糖及其他成分。
J Gen Microbiol. 1966 Sep;44(3):329-41. doi: 10.1099/00221287-44-3-329.
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A study of the immunochemistry of three yeast mannans.三种酵母甘露聚糖的免疫化学研究
J Biol Chem. 1970 Mar 10;245(5):1197-203.
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Method for fingerprinting yeast cell wall mannans.酵母细胞壁甘露聚糖指纹图谱分析方法。
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7
Studies on the antigenic activities of yeasts. II. Isolation and inhibition assay of the oligosaccharides from acetolysate of the mannan of Candida albicans.酵母抗原活性的研究。II. 白色念珠菌甘露聚糖乙酰水解产物中寡糖的分离及抑制试验
Jpn J Microbiol. 1968 Dec;12(4):413-22. doi: 10.1111/j.1348-0421.1968.tb00414.x.
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Mannan as a major component of the bud scars of Saccharomyces cerevisiae.甘露聚糖是酿酒酵母芽痕的主要成分。
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Entrapment of collagen in a polyacrylamide matrix and its application in the purification of animal collagenases.胶原蛋白包埋于聚丙烯酰胺基质及其在动物胶原酶纯化中的应用。
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