Localization of alpha-Galactomannan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy.

Galactomannan was localized by scanning and transmission electron microscopy on the cells and cell walls of Schizosaccharomyces pombe. The markers were prepared from colloidal gold granules labelled with an alpha-galactopyranosyl-binding lectin isolated from the seeds of Bandeiraea simplicifolia. Part or all of this alpha-galactomannan was present in the outer layer of the cell wall and was uniformly distributed even on the fission scars.