Schaller M, Neubert U
Dermatologische Klinik, Ludwig-Maximilians-Universität, München, Germany.
Infection. 1994 Nov-Dec;22(6):401-6. doi: 10.1007/BF01715497.
The aim of this study was to investigate the morphological changes of Borrelia burgdorferi associated with penicillin treatment. An isolate of B. burgdorferi from an erythema migrans lesion was cultivated in BSK II medium and exposed to increasing concentrations (0.0625 mg/l-2 mg/l) of penicillin G for 5 days. The in vitro minimal inhibitory concentration (MIC) was determined to be 0.5 mg/l by broth dilution method. The morphological structures of untreated spirochetes, as well as their characteristic ultrastructural changes when exposed to penicillin, were observed by electron microscopy. The following alterations were discovered: (i) Numerous outer sheath blebs at a penicillin concentration of 0.0625 mg/l. (ii) A characteristic irregular waveform of the borrelial cells and complete loss of the outer sheath at a penicillin concentration of 0.125 mg/l. (iii) The presence of "spheroplasts" at the same concentration. (iv) Structural changes of the protoplasmic cylinder complex which showed an irregular pattern at a penicillin concentration of 0.125 mg/l. (v) Disruption of the protoplasmic cylinder complex into several parts at penicillin concentrations of 0.25 mg/l and 0.5 mg/l. (vi) Severe cytolysis at penicillin concentrations of 1 mg/l and 2 mg/l.
本研究的目的是调查与青霉素治疗相关的伯氏疏螺旋体的形态变化。从游走性红斑病灶分离出的一株伯氏疏螺旋体在BSK II培养基中培养，并暴露于浓度逐渐增加（0.0625 mg/l - 2 mg/l）的青霉素G中5天。通过肉汤稀释法测定体外最小抑菌浓度（MIC）为0.5 mg/l。通过电子显微镜观察未处理的螺旋体的形态结构，以及它们在暴露于青霉素时的特征性超微结构变化。发现了以下改变：（i）青霉素浓度为0.0625 mg/l时出现大量外鞘泡。（ii）青霉素浓度为0.125 mg/l时，伯氏疏螺旋体细胞呈现特征性的不规则波形且外鞘完全丧失。（iii）在相同浓度下出现“原生质球”。（iv）青霉素浓度为0.125 mg/l时，原生质圆柱体复合体的结构变化呈现不规则模式。（v）青霉素浓度为0.25 mg/l和0.5 mg/l时，原生质圆柱体复合体破裂成几个部分。（vi）青霉素浓度为1 mg/l和2 mg/l时出现严重的细胞溶解。
