Passero M A, Tye R W, Kilburn K H, Lynn W S
Proc Natl Acad Sci U S A. 1973 Apr;70(4):973-6. doi: 10.1073/pnas.70.4.973.
The saline-insoluble particulate material obtained by pulmonary lavage from four patients with alveolar proteinosis was analyzed for total lipid (52%), protein (44%), and carbohydrate (4%). Sodium dodecyl sulfate-gel electrophoresis revealed only three major peptides in these patients. Molecular weights (from the gels) were 69,000, 62,000, and 36,000, and the latter two peptides were periodic acid-Schiff stain-positive. Amino-acid and sugar analyses were performed on the peptides cut from the gels and on peptides purified by Sephadex G-200 chromatography. The 69,000-molecular-weight peptide contained no carbohydrate. Antibody studies and aminoacid analysis indicated that it was albumin. The 62,000-molecular-weight peptide contained 1% hydroxyproline, 1% hydroxylysine, 10% glycine, and 9% carbohydrate. The 36,000-molecular-weight peptide contained 1.2% hydroxyproline, 1% hydroxylysine, 13% glycine, 1.4% sialic acid, 2.6% glucose, 2.4% galactose, 2% mannose, 0.8% fucose, 0.4% glycosamine, and 0.6% galactosamine. Proteins extracted from kidney glomeruli with similar amino-acid and carbohydrate composition have been observed previously.
对从四名肺泡蛋白沉积症患者肺灌洗获得的盐水不溶性颗粒物质进行了总脂质（52%）、蛋白质（44%）和碳水化合物（4%）分析。十二烷基硫酸钠-凝胶电泳显示这些患者仅存在三种主要肽段。（根据凝胶测定）分子量分别为69,000、62,000和36,000，后两种肽段对高碘酸-希夫染色呈阳性。对从凝胶上切下的肽段以及通过葡聚糖凝胶G-200色谱法纯化的肽段进行了氨基酸和糖分析。分子量为69,000的肽段不含碳水化合物。抗体研究和氨基酸分析表明它是白蛋白。分子量为62,000的肽段含有1%的羟脯氨酸、1%的羟赖氨酸、10%的甘氨酸和9%的碳水化合物。分子量为36,000的肽段含有1.2%的羟脯氨酸、1%的羟赖氨酸、13%的甘氨酸、1.4%的唾液酸、2.6%的葡萄糖、2.4%的半乳糖、2%的甘露糖、0.8%的岩藻糖、0.4%的氨基葡糖和0.6%的半乳糖胺。先前已观察到从肾小球提取的具有相似氨基酸和碳水化合物组成的蛋白质。
