表达胆碱能和肾上腺素能特性的克隆杂交细胞系。
Clonal hybrid cell lines expressing cholinergic and adrenergic properties.
作者信息
Heumann R, Ocalan M, Kachel V, Hamprecht B
出版信息
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4674-7. doi: 10.1073/pnas.76.9.4674.
Abstract
Different cholinergic cell lines were fused with an adrenergic neuroblastoma cell line (N115-BU-8). Its fusion with a cholinergic neuroblastoma-glioma hybrid produced a "hybrid-hybrid" line containing cholinergic and adrenergic enzyme activities. Both activities were also present in subclones of this line. The presence of catecholamines in single cells was confirmed by microspectrofluorimetry. These results are discussed with respect to the possibility of a simultaneous synthesis of noradrenaline and acetylcholine in single cells. The cholinergic and adrenergic enzyme activities are influenced by cell density, by dexamethasone, and by conditioned medium.
摘要
将不同的胆碱能细胞系与一种肾上腺能神经母细胞瘤细胞系（N115 - BU - 8）进行融合。它与一种胆碱能神经母细胞瘤 - 胶质瘤杂交细胞融合产生了一个含有胆碱能和肾上腺能酶活性的“杂交 - 杂交”细胞系。该细胞系的亚克隆中也存在这两种活性。通过显微分光荧光测定法证实了单细胞中儿茶酚胺的存在。针对单细胞中同时合成去甲肾上腺素和乙酰胆碱的可能性对这些结果进行了讨论。胆碱能和肾上腺能酶活性受细胞密度、地塞米松和条件培养基的影响。
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