Weissfeld A S, Sanner G D, Childress J R, Dyckman J D, Huber T W, Williams R P
Antimicrob Agents Chemother. 1977 Dec;12(6):703-6. doi: 10.1128/AAC.12.6.703.
We have shown that it is possible to screen for penicillinase-producing Neisseria gonorrhoeae directly from primary culture plates. Experiments involving the cocultivation of four genera of beta-lactamase-positive (beta-lac(+)) bacteria and a beta-lactamase-negative (beta-lac(-)) N. gonorrhoeae on modified Thayer-Martin medium indicated that suppressed or inhibited beta-lac(+) bacteria did not give false-positive results when isolated beta-lac(-) colonies of gonococci were tested. Colonies were assayed for beta-lac production by an adaptation of a microacidometric method in which bacteria could be tested before or after the addition of oxidase reagent.
我们已经证明,直接从原代培养平板上筛选产青霉素酶的淋病奈瑟菌是可行的。在改良的Thayer-Martin培养基上,将4属β-内酰胺酶阳性(β-lac(+))细菌与1株β-内酰胺酶阴性(β-lac(-))淋病奈瑟菌共培养的实验表明,当检测分离出的β-lac(-)淋球菌菌落时,受抑制的β-lac(+)细菌不会产生假阳性结果。通过一种微量酸度测定法的改良方法检测菌落的β-lac产生情况,该方法可以在添加氧化酶试剂之前或之后对细菌进行检测。