Martínez R D, Montfort I
Immunology. 1973 Aug;25(2):197-203.
A specific antibody has been prepared in the rabbit against purified rat alveolar macrophages. Immunofluorescent and absorption tests show that rat alveolar macrophages are carriers of specific antigen(s) that are not visible in rat monocytes and free tissue macrophages (peritoneal). On the other hand, rat fixed tissue macrophages from different organs such as liver, spleen, thymus, and lymph node show high percentages of cross-reaction with the antialveolar macrophage antibody, thus revealing the presence of very similar or the same antigen(s). A certain number (about 20 per cent) of bone marrow cells also give positive reactions but their identity as either fixed tissue macrophages or precursors has not been established. A survey of the distribution of rat alveolar macrophage antigen(s) in cells of the mononuclear phagocytic system in four other animal species revealed a high degree of species specificity.
已在兔体内制备了针对纯化大鼠肺泡巨噬细胞的特异性抗体。免疫荧光和吸收试验表明，大鼠肺泡巨噬细胞是特异性抗原的载体，而这些抗原在大鼠单核细胞和游离组织巨噬细胞（腹膜巨噬细胞）中不可见。另一方面，来自肝脏、脾脏、胸腺和淋巴结等不同器官的大鼠固定组织巨噬细胞与抗肺泡巨噬细胞抗体显示出高比例的交叉反应，从而揭示了非常相似或相同抗原的存在。一定数量（约20%）的骨髓细胞也呈阳性反应，但它们作为固定组织巨噬细胞或前体细胞的身份尚未确定。对其他四种动物物种单核吞噬系统细胞中大鼠肺泡巨噬细胞抗原分布的调查显示出高度的物种特异性。
