Chang Y T
Int J Lepr Other Mycobact Dis. 1976 Jan-Jun;44(1-2):50-7.
Techniques to obtain macrophages from various sources of the mouse were reported. The following sources were included: peritoneal exudate, alveolar lavage, blood leucocytes, bone marrow, spleen, liver, lungs, lymph nodes, thymus, thyroid, heart muscle, kidney, and subcutaneous cover glass implants. Human blood macrophages were also included. Long-term cinemicrographic studies revealed sustained good growth of these macrophages. Cell multiplication was detected in all of these cultures except those obtained from the peritoneal exudate. Pure cultures of macrophages were obtained from blood of the mouse and human. Macrophages obtained from other sources were accompanied by some growth of fibroblasts. Methods to eliminate the fibroblasts in cultures were discussed.
已报道了从小鼠不同来源获取巨噬细胞的技术。包括以下来源：腹腔渗出液、肺泡灌洗、血液白细胞、骨髓、脾脏、肝脏、肺、淋巴结、胸腺、甲状腺、心肌、肾脏以及皮下盖玻片植入物。还包括人类血液巨噬细胞。长期电影显微镜研究显示这些巨噬细胞持续良好生长。除了从腹腔渗出液获得的培养物外，在所有这些培养物中均检测到细胞增殖。从小鼠和人类血液中获得了巨噬细胞的纯培养物。从其他来源获得的巨噬细胞伴有一些成纤维细胞生长。讨论了消除培养物中成纤维细胞的方法。
