Sun A M, Parisius W, Healy G M, Vacek I, Macmorine H G
Diabetes. 1977 Dec;26(12):1136-9. doi: 10.2337/diab.26.12.1136.
A unit was constructed that consisted of a core of hollow fibers through which low-molecular-weight substances, such as glucose and insulin, could pass freely but were impermeable to high-molecular-weight proteins, such as antibodies. Islets of Langerhans from normal rats were planted in the space surrounding the fibers, and either blood or nutrient medium was circulated through the fibers themselves. In experiments with animals, the units were attached to the vascular system of diabetic rats and monkeys. Blood glucose concentrations in the rats were reduced to nondiabetic levels within one hour and were maintained for the duration of the experiments. In monkeys the blood glucose level declined from 210 mg./100 ml. to 90 mg./100 ml. in four hours and insulin in the serum rose to 93 muU./ml. in one-half hour. Also, we have found that islets from monkeys cultivated in the artificial endocrine pancreas (AEP) continue to release insulin into circulating tissue culture medium for over eight months.
构建了一个装置，其核心是中空纤维，葡萄糖和胰岛素等低分子量物质可自由通过这些纤维，但抗体等高分子量蛋白质则无法透过。将正常大鼠的胰岛种植在纤维周围的空间中，血液或营养培养基在纤维内部循环流动。在动物实验中，将这些装置连接到糖尿病大鼠和猴子的血管系统。大鼠的血糖浓度在一小时内降至非糖尿病水平，并在实验期间一直维持该水平。猴子的血糖水平在四小时内从210毫克/100毫升降至90毫克/100毫升，血清中的胰岛素在半小时内升至93微单位/毫升。此外，我们还发现，在人工内分泌胰腺（AEP）中培养的猴子胰岛能够在超过八个月的时间里持续向循环组织培养基中释放胰岛素。
