病毒阴性小鼠细胞中C型病毒基因产物的分离与鉴定
Isolation and characterization of C-type viral gene products of virus-negative mouse cells.
作者信息
Stephenson J R, Tronick S R, Reynolds R K, Aaronson S A
出版信息
J Exp Med. 1974 Feb 1;139(2):427-38. doi: 10.1084/jem.139.2.427.
Abstract
Antigens which immunologically cross-react with two mouse C-type viral polypeptides, p30 and p12, are present at very low levels in normal virus-negative mouse cells. These two antigens have been purified by 50-300-fold from cell extracts and shown to cochromatograph with the corresponding labeled viral polypeptides in several systems. Their type-specific antigenicities are shown to be distinct from those of previously tested MuLV isolates suggesting that they may be components of a new class of endogenous C-type virus. The methods utilized in the present studies for concentration of virus-specific antigens of normal mouse cells provide an approach for detection of C-type viral antigens in cells of other species.
摘要
与两种小鼠C型病毒多肽p30和p12发生免疫交叉反应的抗原，在正常的病毒阴性小鼠细胞中含量极低。这两种抗原已从细胞提取物中纯化了50至300倍，并在多个系统中显示与相应的标记病毒多肽共层析。它们的型特异性抗原性与先前测试的莫氏白血病病毒（MuLV）分离株不同，这表明它们可能是一类新型内源性C型病毒的组成部分。本研究中用于浓缩正常小鼠细胞病毒特异性抗原的方法，为检测其他物种细胞中的C型病毒抗原提供了一种途径。
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