Kinsella T D
J Clin Invest. 1974 Apr;53(4):1108-14. doi: 10.1172/JCI107648.
The effect of heat-aggregated human gamma globulin (aggFII) on the induction of in vitro lymphocyte transformation, measured by the uptake of tritiated thymidine into newly synthesized DNA, was studied with peripheral blood lymphocytes derived from 12 patients with rheumatoid arthritis (RA), six with ankylosing spondylitis (AS), two with systemic lupus erythematosus (SLE), and seven normal subjects. It was found that 200 mug aggFII induced significant transformation of the lymphocytes of eight patients with RA, five with AS, one with SLE, and one normal subject. Neither deaggregated FII nor heat-aggregated human serum albumin induced significant transformation of the lymphocytes of any subject tested. A source of complement appeared necessary to support aggFII-induced blastogenesis, since enhanced transformation occurred only in the presence of fresh plasma. Heat-inactivated plasma and fetal calf serum (FCS), and FCS devoid of hemolytic complement, failed to support enhanced blastogenesis in the presence of aggFII. Since substrates similar to those employed in these studies are present in vivo in the rheumatoid joint, it is suggested that aggFII may enhance intra-articular lymphocyte transformation in subjects with RA.
通过测量氚标记胸腺嘧啶核苷掺入新合成DNA的量来研究热聚集人γ球蛋白（aggFII）对体外淋巴细胞转化的诱导作用，研究对象为12例类风湿关节炎（RA）患者、6例强直性脊柱炎（AS）患者、2例系统性红斑狼疮（SLE）患者的外周血淋巴细胞以及7名正常受试者。结果发现，200μg aggFII可诱导8例RA患者、5例AS患者、1例SLE患者以及1名正常受试者的淋巴细胞发生显著转化。未聚集的FII和热聚集人血清白蛋白均未诱导任何受试对象的淋巴细胞发生显著转化。补体来源似乎是支持aggFII诱导的细胞增殖所必需的，因为只有在新鲜血浆存在时才会发生增强的转化。热灭活血浆和胎牛血清（FCS）以及缺乏溶血补体的FCS，在aggFII存在时均无法支持增强的细胞增殖。由于这些研究中使用的类似底物在类风湿关节的体内存在，因此提示aggFII可能增强RA患者关节内的淋巴细胞转化。
