Goldschneider I
J Immunol. 1977 Jun;118(6):2040-6.
Lymphohemopoietic precursor cells in rat bone marrow are members of a subset of lymphocyte-like cells that bears the bone marrow lymphocyte antigen (BMLA) and that lacks antigens present on peripheral B and T cells. This was demonstrated by two experimental approaches. In the first, bone marrow cells with the potential to form hemopoietic colonies in spleen (CFU-S), to repopulate lumphoid tissues and blood, and to rescue lethally irradiated recipients were enriched approximately 10-fold by a fractionation procedure designed to isolate a "null" population of bone marro lymphocytes. In the second approach, the lymphohemopoietic precursor cell activity in bone marrow was completely abrogated by opsonization with rabbit antiserum (ALSBM) raised against this "null" population of bone marrow cells. Precursor cell activity was not affected by treatment with antiserum to T and B cells. Quantitative cross-absorption studies showed that the antigen detected by ALSBM on lymphohemopoietic precursor cells had the same cellular distribution as did the previously described bone marrow lymphocyte antigen. It is likely that this antigen is present both on pluripotent stem cells and on committed progenitors of the myelocytic, erythrocytic and lymphocytic series.
大鼠骨髓中的淋巴细胞造血前体细胞是一类淋巴细胞样细胞亚群的成员，这类细胞带有骨髓淋巴细胞抗原（BMLA），且缺乏外周B细胞和T细胞上存在的抗原。这一点通过两种实验方法得以证明。第一种方法是，通过一种旨在分离骨髓淋巴细胞“空”群体的分级分离程序，将具有在脾脏中形成造血集落（CFU-S）、重新填充淋巴组织和血液以及拯救致死性照射受体潜力的骨髓细胞富集了约10倍。第二种方法是，用针对这种骨髓细胞“空”群体产生的兔抗血清（ALSBM）进行调理作用，完全消除了骨髓中的淋巴细胞造血前体细胞活性。前体细胞活性不受抗T细胞和抗B细胞血清处理的影响。定量交叉吸收研究表明，ALSBM在淋巴细胞造血前体细胞上检测到的抗原与先前描述的骨髓淋巴细胞抗原具有相同的细胞分布。这种抗原很可能同时存在于多能干细胞以及髓细胞系、红细胞系和淋巴细胞系的定向祖细胞上。
