C型病毒中依赖RNA的DNA聚合酶的DNA产物的特异性：3. 对源自叙利亚仓鼠的病毒的分析。
Specificity of the DNA product of RNA-dependent DNA polymerase in type C viruses: 3. Analysis of viruses derived from Syrian hamsters.
作者信息
Okabe H, Gilden R V, Hatanaka M
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3278-82. doi: 10.1073/pnas.71.8.3278.
DNA transcripts were prepared from three related viruses of hamster and analyzed by interviral hybridization and by reaction with cellular DNA. A virus (G-HaLV), isolated from a dimethylbenzanthraceneinduced tumor cell in Graffi hamsters, contained nucleic acid sequences highly specific for hamster cell DNA and did not react with mouse cell DNA nor did its transcript show homology (<5%) with mouse or rat viral RNAs. The hamster-specific sarcoma virus, B-34, isolated by Bassin and coworkers from tumors induced by the Harvey strain of murine sarcoma virus, contained mouse-, hamster-, and possibly rat-specific sequences. B-34 transcripts were predominantly mouse-specific. GLOH(-), a lymphomagenic virus derived by dilution beyond the transforming endpoint of a hamster-specific sarcoma virus obtained from tumors induced by the Gross pseudotype of murine sarcoma virus, also contained hamster- and mouse-specific sequences. Only a portion of its hamster sequence (about 50%) was shared with B-34 and G-HaLV viruses. As expected, transcripts of GLOH(-) virus were reactive with mouse and hamster cellular DNA.
从三种相关的仓鼠病毒制备了DNA转录本，并通过病毒间杂交以及与细胞DNA的反应进行分析。一种从格拉菲仓鼠的二甲基苯并蒽诱导肿瘤细胞中分离出的病毒（G-HaLV），含有对仓鼠细胞DNA高度特异的核酸序列，不与小鼠细胞DNA反应，其转录本也与小鼠或大鼠病毒RNA无同源性（<5%）。仓鼠特异性肉瘤病毒B-34由巴辛及其同事从哈维株小鼠肉瘤病毒诱导的肿瘤中分离得到，含有小鼠、仓鼠以及可能的大鼠特异性序列。B-34转录本主要是小鼠特异性的。GLOH(-)是一种淋巴瘤病毒，通过稀释从由鼠肉瘤病毒格罗斯假型诱导的肿瘤中获得的仓鼠特异性肉瘤病毒的转化终点以上得到，也含有仓鼠和小鼠特异性序列。其仓鼠序列中只有一部分（约50%）与B-34和G-HaLV病毒相同。正如预期的那样，GLOH(-)病毒的转录本与小鼠和仓鼠细胞DNA有反应。
Zotero 插件