Chattopadhyay S K, Lowy D R, Teich N M, Levine A S, Rowe W P
Proc Natl Acad Sci U S A. 1974 Jan;71(1):167-71. doi: 10.1073/pnas.71.1.167.
The AKR mouse has a high titer of murine leukemia virus early in life, and virus-negative cells derived from embryos of this mouse strain can be activated to yield murine leukemia virus by treatment with 5-iododeoxyuridine. In contrast to this high-virus strain, the NIH Swiss mouse has a low incidence of leukemia and no murine leukemia virus has been isolated from it (virus-negative). We have investigated this difference between AKR and NIH mice by examining the sequences specific for murine leukemia virus in nucleic acids of these mice. A single-stranded viral-DNA probe synthesized in vitro using murine-leukemia-virus from the AKR mouse contains at least 87% of the sequences present in the 70S viral RNA; most of these sequences are in proportions similar to their content in the 70S RNA. Using this probe in nucleic acid hybridization experiments, we have shown that NIH-mouse-cell DNA and AKR-mouse-cell DNA differ with respect to sequences specific for AKR murine-leukemia-virus: NIH-mouse-cell DNA lacks some of the virus-specific sequences present in AKR-mouse-cell DNA, and there are two distinct sets of virus-specific sequences in AKR-mouse-cell DNA, whereas there is only one set in NIH-mouse-cell DNA.RNA from virus-negative AKR-mouse cells grown in tissue culture contains some, but not all, virus-specific RNA sequences; however, within 48 hr after initiating treatment of these cells with 5-iododeoxyuridine, the complete viral genome is represented in cellular RNA.
AKR小鼠在生命早期具有高滴度的鼠白血病病毒，并且源自该小鼠品系胚胎的病毒阴性细胞可以通过用5-碘脱氧尿苷处理而被激活以产生鼠白血病病毒。与这种高病毒品系相反，NIH瑞士小鼠白血病发病率低，且未从其分离出鼠白血病病毒（病毒阴性）。我们通过检查这些小鼠核酸中鼠白血病病毒特有的序列，研究了AKR小鼠和NIH小鼠之间的这种差异。使用来自AKR小鼠的鼠白血病病毒体外合成的单链病毒DNA探针包含70S病毒RNA中至少87%的序列；这些序列中的大多数比例与其在70S RNA中的含量相似。在核酸杂交实验中使用该探针，我们发现NIH小鼠细胞DNA和AKR小鼠细胞DNA在AKR鼠白血病病毒特有的序列方面存在差异：NIH小鼠细胞DNA缺乏AKR小鼠细胞DNA中存在的一些病毒特异性序列，并且AKR小鼠细胞DNA中有两组不同的病毒特异性序列，而NIH小鼠细胞DNA中只有一组。在组织培养中生长的病毒阴性AKR小鼠细胞的RNA包含一些但不是全部病毒特异性RNA序列；然而，在用5-碘脱氧尿苷开始处理这些细胞后的48小时内，完整的病毒基因组出现在细胞RNA中。
