通过分子杂交分析C值悖论。
Analysis of the C-value paradox by molecular hybridization.
作者信息
Rosbash M, Ford P J, Bishop J O
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3746-50. doi: 10.1073/pnas.71.9.3746.
Abstract
Poly(A)-containing RNA was isolated from ovaries of Xenopus laevis laevis and Triturus cristatus carnifex and used as a template for the synthesis of radioactive complementary DNA with RNA-dependent DNA polymerase. When annealed with an excess of homologous DNA, the complementary DNA is rendered double-stranded with kinetics that suggest that the coding sequences are single-copy in both these organisms. In Triturus, these sequences are distinct from the majority of the genome, which consists of repeated sequences, and distinct from the ribosomal cistrons, which are present in proportion to the increase in C-value relative to the Xenopus genome. Moreover, the number of different poly(A)-containing molecules in the ovary (sequence complexity) is the same in Xenopus and in Triturus.
摘要
从非洲爪蟾和意大利真螈的卵巢中分离出含多聚腺苷酸（Poly(A)）的RNA，并将其用作模板，用依赖于RNA的DNA聚合酶合成放射性互补DNA。当与过量的同源DNA退火时，互补DNA形成双链的动力学表明，这些编码序列在这两种生物中都是单拷贝的。在意大利真螈中，这些序列与基因组的大部分不同，基因组大部分由重复序列组成，也与核糖体顺反子不同，核糖体顺反子的比例与相对于非洲爪蟾基因组的C值增加成正比。此外，非洲爪蟾和意大利真螈卵巢中不同的含多聚腺苷酸分子数量（序列复杂性）是相同的。
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