Greenleaf A L, Plagens U, Jamrich M, Bautz E K
Chromosoma. 1978 Jan 16;65(2):127-36. doi: 10.1007/BF00329465.
Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff displayed intense fluorescence when stained for RNA polymerase B, but relatively little fluorescence when stained for histone H1. Returning heat shocked larvae to room temperature resulted in the appearance of a distinctive pattern of RNA polymerase-associated fluorescence in the heat shock puff at 87C, presumably reflecting events associated with the inactivation and regression of this puff. Large differences observed in the apparent RNA polymerase B content of puffs of similar size suggest that the interaction of RNA polymerase B with chromosomal loci does not depend on simply the state of condensation or decondensation of the chromatin.
我们运用间接免疫荧光可视化技术，研究了黑腹果蝇多线染色体在热休克期间及之后不同时间，热休克胀泡位点处RNA聚合酶B（或II）和组蛋白H1的分布情况。经过5至45分钟的热处理后，热休克胀泡在使用RNA聚合酶B染色时呈现强烈荧光，但使用组蛋白H1染色时荧光相对较弱。将热休克后的幼虫放回室温，导致在87C的热休克胀泡中出现了一种独特的与RNA聚合酶相关的荧光模式，这可能反映了与此胀泡失活和消退相关的事件。在大小相似的胀泡中观察到的明显RNA聚合酶B含量存在很大差异，这表明RNA聚合酶B与染色体位点的相互作用并不简单取决于染色质的凝聚或解凝聚状态。
