赖氨酸代谢中间体酵母氨酸的生物合成与降解

Biosynthesis and degradation of saccharopine, an intermediate of lysine metabolism.

作者信息

Fellows F C

出版信息

Biochem J. 1973 Oct;136(2):321-7. doi: 10.1042/bj1360321.

DOI:10.1042/bj1360321

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165957/

Abstract

Lysine-2-oxoglutarate reductase was prepared from ox liver and its characteristics were examined. Its activity was totally inhibited in the presence of NH(4)Cl. Under conditions that inhibit saccharopine formation, and in the presence of NADP(+), ox liver mitochondria were found to catalyse the hydrolysis of saccharopine to lysine and alpha-oxoglutarate. The enzyme involved was named saccharopine oxidoreductase. It was partially purified and separated from lysine-oxoglutarate reductase. Comparison of the properties of these two enzymes showed that saccharopine degradation was stimulated under conditions that inhibit its formation. The effect of pH, various cofactors and stability during incubation confirm that saccharopine biosynthesis from, and degradation to, lysine are catalysed by two distinct enzymes.

摘要

从牛肝脏中制备了赖氨酸-2-氧代戊二酸还原酶，并对其特性进行了研究。在氯化铵存在的情况下，其活性被完全抑制。在抑制酵母氨酸形成的条件下，且存在烟酰胺腺嘌呤二核苷酸磷酸（NADP⁺）时，发现牛肝脏线粒体可催化酵母氨酸水解为赖氨酸和α-氧代戊二酸。所涉及的酶被命名为酵母氨酸氧化还原酶。它被部分纯化并与赖氨酸-氧代戊二酸还原酶分离。对这两种酶的特性比较表明，在抑制酵母氨酸形成的条件下，其降解受到刺激。pH值、各种辅因子以及孵育过程中的稳定性的影响证实，赖氨酸的酵母氨酸生物合成和降解是由两种不同的酶催化的。

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本文引用的文献

1

The metabolism of lysine.赖氨酸的代谢

Biochem J. 1944;38(1):119-25. doi: 10.1042/bj0380119.

2

Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。

J Biol Chem. 1951 Nov;193(1):265-75.

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The conversion of lysine to pipecolic acid in the rat.大鼠体内赖氨酸向哌啶酸的转化。

J Biol Chem. 1954 Dec;211(2):851-8.

4

Saccharopine, a product of lysine breakdown by mammalian liver.酵母氨酸，一种哺乳动物肝脏中赖氨酸分解的产物。

Biochem Biophys Res Commun. 1965 Jul 26;20(3):285-90. doi: 10.1016/0006-291x(65)90361-x.

5

Familial hyperlysinemia with lysine-ketoglutarate reductase insufficiency.伴有赖氨酸-酮戊二酸还原酶功能不全的家族性高赖氨酸血症

J Clin Invest. 1969 Aug;48(8):1447-52. doi: 10.1172/JCI106110.

6

Saccharopinuria: a new inborn error of lysine metabolism.尿 saccharopine：赖氨酸代谢的一种新的先天性代谢缺陷。

Nature. 1968 May 18;218(5142):679. doi: 10.1038/218679a0.

7

Lysine metabolism in mammals.哺乳动物中的赖氨酸代谢。

Biochem J. 1973 Oct;136(2):329-34. doi: 10.1042/bj1360329.

8

Saccharopine cleavage by a dehydrogenase of human liver.人肝脏脱氢酶对酵母氨酸的裂解作用。

Biochim Biophys Acta. 1970 May 13;206(2):205-14. doi: 10.1016/0005-2744(70)90104-x.

9

Conversion of lysine to saccharopine by human tissues.人体组织将赖氨酸转化为酵母氨酸。

Biochim Biophys Acta. 1968 Apr 16;158(1):62-9. doi: 10.1016/0304-4165(68)90072-x.

10

Saccharopine, an intermediate of the aminoadipic acid pathway of lysine biosynthesis. IV. Saccharopine dehydrogenase.酵母氨酸，赖氨酸生物合成的氨基己二酸途径的一种中间体。IV. 酵母氨酸脱氢酶。

J Biol Chem. 1966 Jul 25;241(14):3435-40.

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