Shadforth M F, McNaughton D C
Ann Rheum Dis. 1978 Feb;37(1):18-23. doi: 10.1136/ard.37.1.18.
A previous report that C3 activation products are demonstrable in the plasma of subjects with rheumatoid arthritis was reinvestigated using 2-dimensional immunoelectrophoresis. Initially it seemed that results were in agreement, but the occurrence of similar patterns in normal sera and poor reproducibility of results suggested the findings were artefacts. When calcium and magnesium ions were removed with EDTA, C3 activation products were no longer demonstrable in plasma from normal subjects or from patients with rheumatoid arthritis. The results obtained without EDTA in the buffer showed a greater degree of complement activation in patients with rheumatoid arthritis, suggesting that in this condition the serum may have a greater potential ability to bring about complement activation.
此前有报告称，类风湿性关节炎患者血浆中可检测到C3激活产物，本研究使用二维免疫电泳对此进行了重新调查。最初结果似乎一致，但正常血清中出现类似模式以及结果的重现性较差表明这些发现是人为现象。当用乙二胺四乙酸（EDTA）去除钙和镁离子后，正常受试者或类风湿性关节炎患者血浆中均不再能检测到C3激活产物。在缓冲液中不添加EDTA时所获得的结果显示，类风湿性关节炎患者体内补体激活程度更高，这表明在这种情况下，血清可能具有更强的补体激活潜在能力。
