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1
Binding of [14C]erythromycin to Escherichia coli ribosomes.[14C]红霉素与大肠杆菌核糖体的结合
Antimicrob Agents Chemother. 1974 Oct;6(4):474-8. doi: 10.1128/AAC.6.4.474.
2
Effect of erythromycin analogues on binding of [14C]erythromycin to Escherichia coli ribosomes.红霉素类似物对[14C]红霉素与大肠杆菌核糖体结合的影响。
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Correlation of effects of erythromycin analogues on intact bacteria and on [14C]erythromycin binding to Escherichia coli ribosomes.红霉素类似物对完整细菌及[14C]红霉素与大肠杆菌核糖体结合的作用相关性
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Antibiotics as probes of ribosome structure: binding of chloramphenicol and erythromycin to polyribosomes; effect of other antibiotics.抗生素作为核糖体结构的探针：氯霉素和红霉素与多核糖体的结合；其他抗生素的作用
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6
Tight binding of clarithromycin, its 14-(R)-hydroxy metabolite, and erythromycin to Helicobacter pylori ribosomes.克拉霉素、其14-(R)-羟基代谢物以及红霉素与幽门螺杆菌核糖体的紧密结合。
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Quantitative binding of 14C-erythromycin a to E. coli ribosomes.
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Biochemistry. 1977 May 31;16(11):2349-56. doi: 10.1021/bi00630a007.
9
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Binding of erythromycin to Escherichia coli ribosomes.红霉素与大肠杆菌核糖体的结合。
J Antibiot (Tokyo). 1971 May;24(5):302-9. doi: 10.7164/antibiotics.24.302.

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10
Effect of leucomycins and analogues on binding [14C ]erythromycin to Escherichia coli ribosomes.柱晶白霉素及其类似物对[14C]红霉素与大肠杆菌核糖体结合的影响。
Antimicrob Agents Chemother. 1974 Nov;6(5):606-12. doi: 10.1128/AAC.6.5.606.

本文引用的文献

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The stoichiometry of erythromycin binding to ribosomal particles of Staphylococcus aureus.
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Demethylation of erythromycins by rabbit tissues in vitro.
Biochem Pharmacol. 1965 Jul;14(7):1049-58. doi: 10.1016/0006-2952(65)90033-x.
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The intermolecular complex of erythromycin and ribosome.红霉素与核糖体的分子间复合物。
J Mol Biol. 1969 Sep 14;44(2):347-61. doi: 10.1016/0022-2836(69)90180-6.
4
Formation of C14-erythromycin-ribosome complex.C14-红霉素-核糖体复合物的形成。
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5
Binding of erythromycin to Escherichia coli ribosomes.红霉素与大肠杆菌核糖体的结合。
J Antibiot (Tokyo). 1971 May;24(5):302-9. doi: 10.7164/antibiotics.24.302.
6
Two types of binding of erythromycin to ribosomes from antibiotic-sensitive and -resistant Bacillus subtilis 168.红霉素与抗生素敏感及耐药的枯草芽孢杆菌168核糖体的两种结合类型。
J Biol Chem. 1969 Feb 25;244(4):727-35.
7
Sensitivity and resistance to erythromycin in Bacillus subtilis 168: the ribosomal binding of erythromycin and chloramphenicol.枯草芽孢杆菌168对红霉素的敏感性和抗性：红霉素与氯霉素的核糖体结合
Biochim Biophys Acta. 1966 Aug 17;123(2):438-40. doi: 10.1016/0005-2787(66)90301-7.
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Inhibitors of ribosome functions.核糖体功能抑制剂。
Annu Rev Microbiol. 1971;25:487-562. doi: 10.1146/annurev.mi.25.100171.002415.
9
A reversible change in the ability of Escherichia coli ribosomes to bind to erythromycin.大肠杆菌核糖体与红霉素结合能力的可逆变化。
J Mol Biol. 1970 Mar;48(3):511-5. doi: 10.1016/0022-2836(70)90062-8.
10
Studies on the formation of transfer ribonucleic acid-ribosome complexes. 3. The formation of peptide bonds by ribosomes in the absence of supernatant enzymes.转移核糖核酸-核糖体复合物形成的研究。3. 无上清液酶时核糖体形成肽键的过程。
J Biol Chem. 1968 May 25;243(10):2810-20.

[14C]红霉素与大肠杆菌核糖体的结合

Binding of [14C]erythromycin to Escherichia coli ribosomes.

作者信息

Pestka S

出版信息

Antimicrob Agents Chemother. 1974 Oct;6(4):474-8. doi: 10.1128/AAC.6.4.474.

DOI:10.1128/AAC.6.4.474
PMID:4157348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC444674/
Abstract

Erythromycin binding to Escherichia coli ribosomes required K(+) and Mg(2+). Under optimal conditions, the dissociation constant for erythromycin binding to E. coli ribosomes was found to be 1.0 x 10(-8) M and 1.4 x 10(-8) M at 24 C and 5 C, respectively. One molecule of [(14)C]erythromycin was bound to each 70S ribosome at equilibrium. Binding of erythromycin to ribosomes was rapid and reversible. The specific rate constants for the forward and reverse reactions were 1.7 x 10(7) liters per mol per min and 0.15 per min, respectively.

摘要

红霉素与大肠杆菌核糖体的结合需要钾离子（K⁺）和镁离子（Mg²⁺）。在最佳条件下，发现红霉素与大肠杆菌核糖体结合的解离常数在24℃和5℃时分别为1.0×10⁻⁸ M和1.4×10⁻⁸ M。在平衡状态下，每个70S核糖体结合一分子的[¹⁴C]红霉素。红霉素与核糖体的结合迅速且可逆。正向和逆向反应的比速率常数分别为1.7×10⁷升/摩尔/分钟和0.15/分钟。