[14C]红霉素与大肠杆菌核糖体的结合
Binding of [14C]erythromycin to Escherichia coli ribosomes.
作者信息
Pestka S
出版信息
Antimicrob Agents Chemother. 1974 Oct;6(4):474-8. doi: 10.1128/AAC.6.4.474.
Abstract
Erythromycin binding to Escherichia coli ribosomes required K(+) and Mg(2+). Under optimal conditions, the dissociation constant for erythromycin binding to E. coli ribosomes was found to be 1.0 x 10(-8) M and 1.4 x 10(-8) M at 24 C and 5 C, respectively. One molecule of [(14)C]erythromycin was bound to each 70S ribosome at equilibrium. Binding of erythromycin to ribosomes was rapid and reversible. The specific rate constants for the forward and reverse reactions were 1.7 x 10(7) liters per mol per min and 0.15 per min, respectively.
摘要
红霉素与大肠杆菌核糖体的结合需要钾离子（K⁺）和镁离子（Mg²⁺）。在最佳条件下，发现红霉素与大肠杆菌核糖体结合的解离常数在24℃和5℃时分别为1.0×10⁻⁸ M和1.4×10⁻⁸ M。在平衡状态下，每个70S核糖体结合一分子的[¹⁴C]红霉素。红霉素与核糖体的结合迅速且可逆。正向和逆向反应的比速率常数分别为1.7×10⁷升/摩尔/分钟和0.15/分钟。
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