Pestka S, Lemahieu R, Miller P
Antimicrob Agents Chemother. 1974 Oct;6(4):489-91. doi: 10.1128/AAC.6.4.489.
The dissociation constants for binding to ribosomes from Escherichia coli and concentrations at which 50% inhibition of [(14)C]erythromycin binding to ribosomes occurred were determined for 45 erythromycin analogues. These values were correlated with their antibacterial activities against Bacillus subtilis. Compounds which bound to ribosomes best showed the greatest activities; those which were poorly bound to ribosomes showed little or no antibacterial activity. The ribosomal binding assays therefore reflected the general antibacterial potential of the erythromycin analogues.
测定了45种红霉素类似物与大肠杆菌核糖体结合的解离常数以及对[¹⁴C]红霉素与核糖体结合产生50%抑制作用时的浓度。这些值与它们对枯草芽孢杆菌的抗菌活性相关。与核糖体结合最佳的化合物显示出最大的活性；与核糖体结合不佳的化合物显示出很少或没有抗菌活性。因此，核糖体结合试验反映了红霉素类似物的总体抗菌潜力。
