Marengo P B, Wilkins J, Overturf G D
Antimicrob Agents Chemother. 1974 Oct;6(4):498-500. doi: 10.1128/AAC.6.4.498.
The emergence of a strain of Providencia stuartii resistant to multiple antibiotics with the exception of amikacin provided a test organism for a microbiological assay for this new semisynthetic aminoglycosidic aminocyclitol. Results can be read at 4 h and are specific for amikacin. The resistance of P. stuartii to all currently used antibiotics allows the utilization of this technique in the presence of other concurrently administered antibiotics and therefore eliminates the need for their inactivation in the assay procedure. The rapidity, specificity, and simplicity of this microbiological assay may provide a technique for routine clinical monitoring of patients on therapeutic regimens and could be utilized by institutions unable to perform the radioimmunoassay or radioassay techniques.
除丁胺卡那霉素外，对多种抗生素耐药的斯氏普罗威登斯菌菌株的出现，为这种新型半合成氨基糖苷类氨基环醇的微生物学测定提供了一种测试生物体。结果可在4小时读取，且对丁胺卡那霉素具有特异性。斯氏普罗威登斯菌对所有目前使用的抗生素的耐药性使得该技术可在同时使用其他抗生素的情况下应用，因此无需在测定过程中使其失活。这种微生物学测定的快速性、特异性和简便性可能为接受治疗方案的患者提供一种常规临床监测技术，并且可供无法进行放射免疫测定或放射测定技术的机构使用。
