Mongar J L, Perera B A
Immunology. 1965 May;8(5):511-8.
The oxygen consumption of isolated rat mast cells has been determined using a micro capillary respirometer designed by Cunningham and Kirk and modified to permit stirring and addition of reactants. Uptake ranged from 0.5 pl. per cell per minute in 100 per cent O to 0.1 pl. per cell per minute in 1 per cent O. It remained steady for at least 1 hour and was not affected by the presence of glucose. Under a variety of conditions neither histamine release by antigen added to sensitized cells nor by compound 48/80 added to normal cells resulted in any increase in the rate of oxygen uptake. Oxygen does not appear to be a rate limiting factor.
使用由坎宁安和柯克设计并经过改进以允许搅拌和添加反应物的微量毛细管呼吸计，测定了分离的大鼠肥大细胞的耗氧量。耗氧量范围为：在100%氧气中，每细胞每分钟0.5皮升；在1%氧气中，每细胞每分钟0.1皮升。它至少保持稳定1小时，且不受葡萄糖存在的影响。在各种条件下，添加到致敏细胞中的抗原或添加到正常细胞中的化合物48/80所引起的组胺释放，均未导致氧气摄取速率的任何增加。氧气似乎不是一个限速因素。
