Armstrong D, Henle G, Henle W
J Bacteriol. 1966 Mar;91(3):1257-62. doi: 10.1128/jb.91.3.1257-1262.1966.
Armstrong, Donald (Children's Hospital of Philadelphia, Philadelphia, Pa.), Gertrude Henle, and Werner Henle. Complement-fixation tests with cell lines derived from Burkitt's lymphoma and acute leukemias. J. Bacteriol. 91:1257-1262. 1966.-Cells of various lines isolated from Burkitt's lymphomas and acute leukemias and disintegrated by freezing and thawing or sonic treatment were found to react in complement-fixation tests with a considerable proportion of human sera. At least 10(7) cells per milliliter were required for antigenic activity. All but one of 13 sera from Burkitt lymphoma patients were positive, with titers ranging from 1:8 to 1:320. About 20% of sera from American children and 60% of sera from adults, regardless of diagnosis, showed titers in a similar range. Sera giving positive tests with one of the neoplastic white cell antigens usually reacted also with many if not all of the others, but rarely with antigens derived from normal peripheral leukocyte cultures and not at all with HeLa or other human nonleukocytic cells. Various observations indicate that the complement-fixation test measures mainly antigens which are different from those detected by immunofluorescence. The nature of the reactions described remains obscure.
阿姆斯特朗，唐纳德（宾夕法尼亚州费城儿童医院）、格特鲁德·亨勒和维尔纳·亨勒。用源自伯基特淋巴瘤和急性白血病的细胞系进行补体结合试验。《细菌学杂志》91:1257 - 1262。1966年。——从伯基特淋巴瘤和急性白血病中分离出的各种细胞系，经冻融或超声处理使其解体后，发现它们在补体结合试验中能与相当一部分人血清发生反应。抗原活性至少需要每毫升10(7)个细胞。13份伯基特淋巴瘤患者的血清中，除1份外均为阳性，滴度范围为1:8至1:320。无论诊断如何，约20%的美国儿童血清和60%的成人血清滴度在类似范围内。与一种肿瘤白细胞抗原呈阳性反应的血清通常也会与许多（如果不是全部）其他抗原发生反应，但很少与源自正常外周白细胞培养物的抗原发生反应，与海拉细胞或其他人类非白细胞细胞则完全不发生反应。各种观察结果表明，补体结合试验主要检测的抗原与免疫荧光检测到的抗原不同。所述反应的性质仍不清楚。
