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使用改良纽约市(MNYC)培养基对淋病进行培养诊断。

Cultural diagnosis of gonorrhoea with modified New York City (MNYC) medium.

作者信息

Young H

出版信息

Br J Vener Dis. 1978 Feb;54(1):36-40. doi: 10.1136/sti.54.1.36.

Abstract

A simply prepared modified New York City medium, designated MNYC was compared with Thayer Martin (TM) medium for the cultural diagnosis of gonorrhoea. MNYC medium contained lincomycin, commercial gonococcal base and lysed whole blood, whereas the original New York City medium contained fresh horse plasma and haemoglobin solution, a basal medium prepared from basic ingredients and vanocmycin. Using MNYC medium gonococci were cultured from 96.1% of men and 100% of women with gonorrhoea (positive film and/or culture) compared with only 77.6% and 69% respectively using TM medium. There were no patients positive by culture on TM medium but negative by culture on MNYC medium. The proportion of men with positive films but negative culture was reduced from 17.1% on TM medium to 3.9% on MNYC medium. There were no women with positive films but negative cultures on MNYC medium compared with 19% on TM medium. MNYC medium is recommended as a simply prepared and highly efficient medium for the cultural diagnosis of gonorrhoea.

摘要

一种制备简单的改良纽约市培养基(称为MNYC)与塞耶-马丁(TM)培养基用于淋病的培养诊断进行了比较。MNYC培养基含有林可霉素、市售淋球菌基础培养基和裂解全血,而原始的纽约市培养基含有新鲜马血浆和血红蛋白溶液、由基本成分制备的基础培养基以及万古霉素。使用MNYC培养基,96.1%的男性淋病患者和100%的女性淋病患者(涂片阳性和/或培养阳性)培养出淋球菌,而使用TM培养基时,这一比例分别仅为77.6%和69%。在TM培养基上培养阴性但在MNYC培养基上培养阳性的患者不存在。男性涂片阳性但培养阴性的比例从TM培养基上的17.1%降至MNYC培养基上的3.9%。MNYC培养基上不存在涂片阳性但培养阴性的女性患者,而TM培养基上这一比例为19%。MNYC培养基被推荐为一种制备简单且高效的淋病培养诊断培养基。

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