枯草芽孢杆菌基因的电泳分离
Electrophoretic separation of Bacillus subtilis genes.
作者信息
Harris-Warrick R M, Elkana Y, Ehrlich S D, Lederberg J
出版信息
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207-11. doi: 10.1073/pnas.72.6.2207.
Abstract
The cleavage of Bacillus subtilis DNA by EcoR1 restriction endonuclease produced segments which retain various degrees of genetic transforming activity. The active segments analyzed thus far, range in size from 23 to 3 kilobases and can be partially separated by agarose gel electrophoresis. Various markers can thus be enriched from 30- to 60-fold.
摘要
用EcoR1限制性内切酶切割枯草芽孢杆菌DNA产生的片段保留了不同程度的遗传转化活性。迄今为止分析的活性片段大小在23至3千碱基之间，并且可以通过琼脂糖凝胶电泳进行部分分离。因此，各种标记物可以富集30至60倍。
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