Sassen A, Perkins E H, Brown R A
Immunology. 1968 Feb;14(2):247-56.
Heat-treated, but not untreated, human γ-globulin (HGG) is a potent antigen for the initiation of primary antibody response in mice. Thus a dose as low as 10 g of heat-treated HGG was sufficient to induce an excellent primary response in intact mice. However, the primary response was meagre and negative in the cell-transfer and diffusion-chamber culture systems of dispersed spleen cells, respectively. In contrast, no significant difference could be demonstrated between heat-treated and untreated HGG preparations in their capacity to induce a secondary response in intact mice. Furthermore, good secondary responses were obtained in both the cell-transfer and diffusion-chamber culture systems. This suggests differences between primed and non-primed spleen cells in their requirements of cellular organization for the initiation of antibody response. Both the primary and the secondary responses were found to be antigen—dose dependent, although the optimum antigen dose range was broad. Moreover, doses of heat-treated HGG (≤10 g) lower than that reported for the excellent immunogen, flagella, were sufficient to induce secondary responses, thereby emphasizing the utility of HGG for studying the role of antigen in the initiation of primary and secondary antibody responses.
经热处理而非未经处理的人γ球蛋白（HGG）是在小鼠中引发初次抗体应答的有效抗原。因此，低至10微克的经热处理的HGG剂量就足以在完整小鼠中诱导出良好的初次应答。然而，在分散脾细胞的细胞转移和扩散室培养系统中，初次应答分别很微弱且呈阴性。相比之下，经热处理和未经处理的HGG制剂在诱导完整小鼠二次应答的能力上没有显著差异。此外，在细胞转移和扩散室培养系统中均获得了良好的二次应答。这表明已致敏和未致敏的脾细胞在引发抗体应答所需的细胞组织要求方面存在差异。尽管最佳抗原剂量范围较宽，但初次应答和二次应答均发现是抗原剂量依赖性的。此外，经热处理的HGG（≤10微克）的剂量低于已报道的优秀免疫原鞭毛的剂量，却足以诱导二次应答，从而强调了HGG在研究抗原在初次和二次抗体应答引发中的作用方面的实用性。
