Macario A J, Conway de Macario E
Clin Exp Immunol. 1978 Feb;31(2):281-90.
A new experimental system is described which allows the study of the effect of immunosuppressors upon the priming and generation of memory to an antigen from Escherichia coli. A single dose of bacterial beta-D-galactosidase without adjuvant injected into C57B1/6J mice primes and elicits memory but not antibodies. Thus by administering immunosuppressors near the priming injection, one can examine whether primary antibody formation is enhanced and whether priming generation of memory is enhanced or inhibited. We found that X-rays, cyclophosphamide and oxisuran (2-[(methylsulfinyl)acetyl]pyridine) either enhance or inhibit the elicitation of memory, depending on dosage, although they do not alter primary antibody unresponsiveness. The data show two main features: (a) immunosuppressors can enhance immunization; and (b) generation of memory can be improved without increasing antibody levels. The former finding draws attention to the role that immunosuppressors might play in the breaching of tolerance to self-antigens which share determinants with microbes, while the latter observation shows that antibody synthesis and elicitation of memory can follow independent pathways.
本文描述了一种新的实验系统，该系统可用于研究免疫抑制剂对针对大肠杆菌抗原的致敏作用及记忆产生的影响。将无佐剂的单剂量细菌β-D-半乳糖苷酶注射到C57B1/6J小鼠体内可引发并诱导记忆，但不会产生抗体。因此，通过在致敏注射附近给予免疫抑制剂，人们可以研究初次抗体形成是否增强，以及记忆的致敏产生是增强还是受到抑制。我们发现，X射线、环磷酰胺和氧异环磷酰胺（2-[(甲基亚磺酰基)乙酰基]吡啶）根据剂量不同，要么增强要么抑制记忆的诱导，尽管它们不会改变初次抗体无反应性。数据显示出两个主要特征：（a）免疫抑制剂可增强免疫；（b）在不增加抗体水平的情况下可改善记忆的产生。前一个发现使人们关注免疫抑制剂在突破对与微生物共享决定簇的自身抗原的耐受性中可能发挥的作用，而后一个观察结果表明抗体合成和记忆诱导可遵循独立的途径。
