Chappel R J, McNaught D J, Bourke J A, Allan G S
J Hyg (Lond). 1978 Jun;80(3):365-71. doi: 10.1017/s0022172400024815.
A total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW),the serum agglutination test (SAT) and the radioimmunoassay (RIA). The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody. Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.
总共1887份对玫瑰红平板试验呈阳性的牛血清接受了其他牛布鲁氏菌病血清学检测:采用温育固定法的补体结合试验(CFTW)、血清凝集试验(SAT)和放射免疫测定(RIA)。SAT通常比CFTW敏感性低得多。然而,许多血清在SAT中呈阳性反应,但在CFTW或RIA中无反应。这些SAT反应归因于IgM抗体。CFTW和RIA结果的比较得出结论,200 ng可作为RIA中的最低诊断反应。