Lovett P S, Young F E
J Bacteriol. 1969 Nov;100(2):658-61. doi: 10.1128/jb.100.2.658-661.1969.
The physiological and biochemical properties of a species of Bacillus previously identified as B. subtilis NRRL B-3275 (B-3275) were compared with those of seven strains of B. pumilus and five strains of B. subtilis. The biotin requirement of B-3275, its inability to hydrolyze starch, and its failure to reduce nitrate indicate that the organism is more closely related to the B. pumilus strains than to those of B. subtilis. Hybridization of deoxyribonucleic acid (DNA) from B-3275 with that of the strains of B. pumilus showed a binding efficiency (compared with the homologous reaction) of 58 to 99%, depending on the strain. Hybridization with the DNA from any of the strains of B. subtilis did not exceed 24%. DNA from B-3275 was unable to transform two amino acid auxotrophic markers to prototrophy in a highly competent strain of B. subtilis 168. We conclude that B-3275 is a strain of B. pumilus which we designate as B. pumilus NRRL B-3275.
将先前鉴定为枯草芽孢杆菌NRRL B - 3275（B - 3275）的一种芽孢杆菌的生理生化特性与7株短小芽孢杆菌菌株和5株枯草芽孢杆菌菌株的特性进行了比较。B - 3275对生物素的需求、其无法水解淀粉以及不能还原硝酸盐，表明该生物体与短小芽孢杆菌菌株的亲缘关系比与枯草芽孢杆菌菌株的亲缘关系更近。来自B - 3275的脱氧核糖核酸（DNA）与短小芽孢杆菌菌株的DNA杂交显示，结合效率（与同源反应相比）为58%至99%，具体取决于菌株。与任何枯草芽孢杆菌菌株的DNA杂交均未超过24%。来自B - 3275的DNA无法在枯草芽孢杆菌168的高感受态菌株中将两个氨基酸营养缺陷型标记转化为原养型。我们得出结论，B - 3275是一株短小芽孢杆菌，我们将其命名为短小芽孢杆菌NRRL B - 3275。
