Roberts R B
J Exp Med. 1970 Mar 1;131(3):499-513. doi: 10.1084/jem.131.3.499.
The interaction in vitro between human granulocytes and meningococci in the presence of sera from volunteers immunized by Gotschlich et al. with purified group A and group C meningococcal polysaccharides was studied. Phagocytosis of meningococci did not occur in the presence of preimmunization sera. In all volunteers tested, group-specific opsonins were detected in groups A and C polysaccharide antisera. Opsonic activity appeared within 1 wk after immunization and persisted for at least 14 months. Titers of opsonic activity ranged from 1:20 to 1:320; highest titers were noted in 2-4 wk antisera. Meningococcal opsonins were detected in both 19S and 7S immunoglobulins. Opsonic activity in low-titer antisera depended on heat-labile factors present in both normal and immune sera, whereas phagocytosis was observed in the presence of heat-inactivated high-titer antisera. Phagocytosis of group A meningococci in the presence of certain group A polysaccharide antisera was inhibited by N-acetyl mannosamine, but not by mannose, mannosamine, N-acetyl glucosamine, N-acetyl galactosamine, or N-acetyl neuraminic acid. Absorption studies with sera from patients with natural meningococcal infections revealed that these polysaccharides are the major antiphagocytic determinants for group A and group C meningococci. These studies are consistent with previous reports suggesting that immunization with group A and group C polysaccharides may well provide group-specific protection against meningococcal infections.
研究了在戈奇利希等人用纯化的A群和C群脑膜炎球菌多糖免疫的志愿者血清存在下，人粒细胞与脑膜炎球菌的体外相互作用。在免疫前血清存在的情况下，脑膜炎球菌的吞噬作用未发生。在所有测试的志愿者中，在A群和C群多糖抗血清中检测到群特异性调理素。调理活性在免疫后1周内出现，并持续至少14个月。调理活性滴度范围为1:20至1:320；在2-4周的抗血清中观察到最高滴度。在19S和7S免疫球蛋白中均检测到脑膜炎球菌调理素。低滴度抗血清中的调理活性取决于正常血清和免疫血清中存在的热不稳定因子，而在热灭活的高滴度抗血清存在的情况下观察到吞噬作用。在某些A群多糖抗血清存在下，A群脑膜炎球菌的吞噬作用受到N-乙酰甘露糖胺的抑制，但不受甘露糖、甘露糖胺、N-乙酰葡糖胺、N-乙酰半乳糖胺或N-乙酰神经氨酸的抑制。对自然感染脑膜炎球菌患者血清的吸收研究表明，这些多糖是A群和C群脑膜炎球菌的主要抗吞噬决定因素。这些研究与先前的报告一致，表明用A群和C群多糖免疫很可能提供针对脑膜炎球菌感染的群特异性保护。
