Kozel T R, Follette J L
Infect Immun. 1981 Mar;31(3):978-84. doi: 10.1128/iai.31.3.978-984.1981.
Antisera prepared in rabbits against either whole encapsulated cells of Cryptococcus neoformans or purified cryptococcal polysaccharide were opsonic for the encapsulated yeast. The opsonic activity was removed by absorption with whole cryptococci and was inhibited by free polysaccharide. As little as 0.13 microgram of cryptococcal polysaccharide produced a 50% inhibition of opsonization. Various degrees of neutralization by polysaccharides from the four cryptococcal serotypes suggested that the opsonins were type specific. Fractionation of antiserum on Bio-Gel A-5m (Bio-Rad Laboratories) and diethylaminoethyl cellulose showed that the opsonins were antibodies of the immunoglobulin G class. These opsonizing antibodies did not require heat-labile serum components for optimal phagocytosis of the yeast. Inhibition studies using 2-deoxy-D-glucose demonstrated that ingestion of encapsulated cryptococci opsonized with anticapsular antibody was a 2-deoxy-D-glucose-inhibitable process. This result differed from similar studies with non-encapsulated cryptococci which showed that ingestion of non-encapsulated cryptococci opsonized with normal serum was not inhibited by 2-deoxy-D-glucose.
用兔制备的抗血清，无论是针对新型隐球菌的全包膜细胞还是纯化的隐球菌多糖，对包膜酵母均有调理作用。调理活性可通过与全隐球菌吸收而去除，并被游离多糖抑制。低至0.13微克的隐球菌多糖可产生50%的调理抑制作用。来自四种隐球菌血清型的多糖的不同程度的中和作用表明调理素具有型特异性。在Bio-Gel A-5m（伯乐公司）和二乙氨基乙基纤维素上对抗血清进行分级分离表明，调理素是免疫球蛋白G类抗体。这些调理抗体在酵母的最佳吞噬作用中不需要热不稳定的血清成分。使用2-脱氧-D-葡萄糖的抑制研究表明，摄入用抗荚膜抗体调理的包膜隐球菌是一个2-脱氧-D-葡萄糖可抑制的过程。这一结果与对非包膜隐球菌的类似研究不同，后者表明摄入用正常血清调理的非包膜隐球菌不受2-脱氧-D-葡萄糖抑制。
