Camyre K P, Pfau C J
J Virol. 1968 Mar;2(3):161-6. doi: 10.1128/JVI.2.3.161-166.1968.
Biological, biochemical, and biophysical properties of three lymphocytic choriomeningitis (LCM) virus strains were compared. The biological property examined was the concentration range of virus which would, when injected into neonates, cause a carrier state. The dosage range for the CA1371 and Traub strains was found to be as broad as the limits examined (5 to 100 ld(50) units/mouse). The WCP strain, however, would only produce carriers within a 3 to 5 ld(50) range. The biochemical properties examined were the growth rates in tissue culture and the effect of varying the input ratio of virus to cells. With identical input ratios, the Traub strain reached a peak titer 32 hr after infection. The CA1371 and WCP strain reached their peaks at the 40th hr. With a 10-fold decrease in the amount of CA1371 virus per cell, peak titer (as high as in the above experiments) was not obtained until 56 hr postinfection. The biophysical properties examined were stability in density gradients and inactivation rates at 4C. In potassium tartrate gradients, full recovery of the CA1371 and WCP strain could be achieved. However, inactivation kinetics showed that only the CA1371 strain was much more stable than the Traub-LCM. The realization that marked differences in LCM strains exist is discussed in relation to certain inconsistencies in the literature.
对三种淋巴细胞性脉络丛脑膜炎（LCM）病毒株的生物学、生物化学和生物物理特性进行了比较。所检测的生物学特性是注入新生动物后会导致携带状态的病毒浓度范围。发现CA1371株和特劳伯株的剂量范围与所检测的极限一样宽（5至100个半数致死量单位/小鼠）。然而，WCP株仅在3至5个半数致死量范围内产生携带状态。所检测的生物化学特性是在组织培养中的生长速率以及改变病毒与细胞输入比例的影响。在相同的输入比例下，特劳伯株在感染后32小时达到滴度峰值。CA1371株和WCP株在第40小时达到峰值。当每个细胞中CA1371病毒量减少10倍时，直到感染后56小时才获得峰值滴度（与上述实验中的滴度一样高）。所检测的生物物理特性是在密度梯度中的稳定性和在4℃下的失活速率。在酒石酸钾梯度中，CA1371株和WCP株能够完全恢复。然而，失活动力学表明只有CA1371株比特劳伯 - LCM株稳定得多。结合文献中的某些不一致之处，讨论了LCM病毒株存在显著差异这一认识。
